Human fetal intestinal epithelial cells metabolize and incorporate branched chain fatty acids in a structure specific manner.
Human fetal intestinal epithelial cells metabolize and incorporate branched chain fatty acids in a structure specific manner.
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人类胎儿肠上皮细胞以特定方式代谢并掺入分支链脂肪酸。
DOI：
10.1016/j.plefa.2016.12.002
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Brenna JT
中科院分区：
文献类型：
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作者：
Liu L;Wang Z;Park HG;Xu C;Lawrence P;Su X;Wijendran V;Walker WA;Kothapalli KS;Brenna JT
支链脂肪酸（BCFA）是健康新生儿胃肠道（GI）的组成部分，在新生大鼠模型中降低坏死性小肠结肠炎（NEC）的发生率，并在体内被纳入小肠细胞脂质中。我们假设BCFA在体外被人体胎儿细胞吸收、代谢并结合。将人H4细胞（一种胎儿未转化的原代小肠细胞系）与白蛋白结合的非酯化前iso-17:0、iso-16:0、iso-18:0和/或iso-20:0培养，分析脂质部分的FA谱。所有BCFA都很容易被纳入细胞脂质的主要成分。在BCFA测试中，Anteiso-17:0在取代正常（n-） FA方面效果最好。iso BCFA的优先顺序与链长相反，iso-20:0出现在最低水平。BCFA掺入磷脂（PL）遵循相同的优先顺序，与BCFA积累42%的FA，没有明显的细胞死亡形态学迹象。尽管胆固醇酯（CE）在所检测的脂类中处于较低的细胞浓度，但CE对BCFA的亲和力最大，积累了65%的FA作为BCFA。BCFA最有效地取代了低饱和FA。Iso-16:0、iso-18:0和前iso-17:0均延长，链缩短±C2。Iso-20:0被链缩短为iso-18:0和iso-16:0，但没有延长。未转化的人胎儿肠上皮细胞在可用时含有高水平的BCFA，并以特定的结构方式代谢它们。这些发现表明，处理BCFA的特定途径存在于妊娠后期暴露于皮脂源性BCFA的人胎儿胃肠道的腔面细胞中。
Branched chain fatty acids (BCFA) are constituents of gastrointestinal (GI) tract in healthy newborn human infants, reduce the incidence of necrotizing enterocolitis (NEC) in a neonatal rat model, and are incorporated into small intestine cellular lipids in vivo. We hypothesize that BCFA are taken up, metabolized and incorporated into human fetal cells in vitro. Human H4 cells, a fetal non-transformed primary small intestine cell line, were incubated with albumin-bound non-esterified anteiso-17:0, iso-16:0, iso-18:0 and/or iso-20:0, and FA profiles in lipid fractions were analyzed. All BCFA were readily incorporated as major constituents of cellular lipids. Anteiso-17:0 was preferentially taken up, and was most effective among BCFA tested in displacing normal (n-) FA. The iso BCFA were preferred in reverse order of chain length, with iso-20:0 appearing at lowest level. BCFA incorporation in phospholipids (PL) followed the same order of preference, accumulating 42% of FA as BCFA with no overt morphological signs of cell death. Though cholesterol esters (CE) are at low cellular concentration among lipid classes examined, CE had the greatest affinity for BCFA, accumulating 65% of FA as BCFA. BCFA most effectively displaced lower saturated FA. Iso-16:0, iso-18:0 and anteiso-17:0 were both elongated and chain shortened by ± C2. Iso-20:0 was chain shortened to iso-18:0 and iso-16:0 but not elongated. Nontransformed human fetal intestinal epithelial cells incorporate high levels of BCFA when they are available and metabolize them in a structure specific manner. These findings imply that specific pathways for handling BCFA are present in the lumen-facing cells of the human fetal GI tract that is exposed to vernix-derived BCFA in late gestation.