α-Glucan phosphorylase and its use in carbohydrate engineering
α-Glucan phosphorylase and its use in carbohydrate engineering
复制标题
DOI：
10.1002/jsfa.2513
复制
发表时间：
2006-08-30
影响因子：
4.1
通讯作者：
Kuriki, Takashi
中科院分区：
文献类型：
--
作者：
Yanase, Michiyo;Takaha, Takeshi;Kuriki, Takashi
直链淀粉有望成为多种行业的功能性生物材料，但由于从淀粉中纯化天然直链淀粉很困难，因此尚未实现工业规模生产。作者最近建立了一个系统，利用 α-葡聚糖磷酸化酶 (EC 2.4.1.1) 与蔗糖磷酸化酶 (EC 2.4.1.7) 或纤维二糖磷酸化酶 (EC 2.4.1.20) 的联合作用，从蔗糖或纤维二糖生产合成直链淀粉。在这些组合酶系统中，α-葡聚糖磷酸化酶是关键酶。本文总结了作者为工业过程设计实用的α-葡聚糖磷酸化酶的工作及其在线性直链淀粉和其他葡萄糖聚合物的酶促合成中的应用。 (c) 2006年化学工业学会
Amylose is expected to be a functional biomaterial for various industries but is not produced on an industrial scale, since the purification of natural amylose from starch is difficult. The authors have recently established a system to produce synthetic amylose from either sucrose or cellobiose using the combined action of alpha-glucan phosphorylase (EC 2.4.1.1) together with sucrose phosphorylase (EC 2.4.1.7) or cellobiose phosphorylase (EC 2.4.1.20). In these combined enzymic systems, alpha-glucan phosphorylase is a key enzyme. This paper summarises the authors' work to engineer practical alpha-glucan phosphorylase for industrial processes and its use in the enzymatic synthesis of essentially linear amylose and other glucose polymers. (c) 2006 Society of Chemical Industry