Apoptotic-like changes in equine spermatozoa separated by density-gradient centrifugation or after cryopreservation
Apoptotic-like changes in equine spermatozoa separated by density-gradient centrifugation or after cryopreservation
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DOI：
10.1016/j.theriogenology.2008.01.014
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发表时间：
2008-06-01
期刊：
影响因子：
2.8
通讯作者：
Ball, B. A.
中科院分区：
文献类型：
--
作者：
Brum, A. M.;Sabeur, K.;Ball, B. A.
目的：评价马精子密度梯度离心法分离和冷冻保存后精液中的凋亡标志物。Percoll分离的马精子亚群在caspase激活的活精子百分率(2.9+/-0.7%vs14.2+/-6.4%；平均+/-S.E.M.)、低线粒体膜电位(Mp；细胞质膜通透性改变(1.3+/-0.2vs3.0+/-0.5)、DNA片段化(2.0+/-1.3vs14.3+/-3.6)、总运动能力(81.8+/-3.3vs35.1+/-5.4)和前进运动能力(66.3+/-4.3vs24.1+/-4.5)。高密度亚群和低密度亚群之间磷脂酰丝氨酸外化差异无统计学意义(P=0.67)(2.6+/-0.7vs3.1+/-0.9)。马精子冷冻保存后与冷冻前相比差异有统计学意义(P<；Caspase活性百分率(19.1+/-1.6vs52.1+/-2.8)，低基质金属蛋白酶(18.2+/-2.5vs48.7+/-2.6)，质膜通透性改变(6.8+/-1.7vs17.6+/-2.0)，总动力(75.5+/-2.4vs45.2+/-5.6)，冷冻前精子与冷冻精子的前向活动率分别为53.9+/-3.1和28.3+/-4.5。DNA片段化细胞百分率冷冻前(5.5+/-1.2)与冷冻后(6.6+/-1.1)差异无统计学意义(P=0.21)。我们的结论是，在马的射出精子中可以检测到凋亡样变化，并且在冷冻后更为普遍。(C)2008 Elsevier Inc.保留所有权利。
The objective was to evaluate apoptotic markers in ejaculated equine spermatozoa after separation by density-gradient centrifugation and after cryopreservation. Subpopulations of percoll-separated equine spermatozoa differed (P < 0.05) in the percentage of live, caspase-activated spermatozoa (2.9 +/- 0.7% vs 14.2 +/- 6.4%; mean +/- S.E.M.), low mitochondrial membrane potential (MMP; 6.8 +/- 1.1 vs 23.8 +/- 3.7), altered plasma membrane permeability (1.3 +/- 0.2 vs 3.0 +/- 0.5), DNA fragmentation (2.0 +/- 1.3 vs 14.3 +/- 3.6), total motility (81.8 +/- 3.3 vs 35.1 +/- 5.4), and progressive motility (66.3 +/- 4.3 vs 24.1 +/- 4.5) for high-density versus low-density subpopulations, respectively. Phosphatidylserine externalization did not differ (P = 0.67) between the high- and low-density subpopulations (2.6 +/- 0.7 vs 3.1 +/- 0.9). After cryopreservation, equine spermatozoa differed (P < 0.01) in the percentage of active caspases (19.1 +/- 1.6 vs 52.1 +/- 2.8), low MMP (18.2 +/- 2.5 vs 48.7 +/- 2.6), altered plasma membrane permeability (6.8 +/- 1.7 vs 17.6 +/- 2.0), total motility (75.5 +/- 2.4 vs 45.2 +/- 5.6), and progressive motility (53.9 +/- 3.1 vs 28.3 +/- 4.5) for pre-freeze versus cryopreserved spermatozoa. There was no difference (P = 0.2 1) in percentage of DNA fragmented cells before (5.5 +/- 1.2) versus after cryopreservation (6.6 +/- 1.1). We concluded that apoptotic-like changes were detectable in ejaculated equine spermatozoa and were more prevalent after cryopreservation. (C) 2008 Elsevier Inc. All rights reserved.