CHARACTERISTICS OF 2 NEW AVIAN TUMOR VIRUS SUBGROUPS
CHARACTERISTICS OF 2 NEW AVIAN TUMOR VIRUS SUBGROUPS
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DOI：
10.1016/0042-6822(69)90344-4
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发表时间：
1969-01-01
期刊：
影响因子：
3.7
通讯作者：
VOGT, PK
中科院分区：
文献类型：
--
作者：
DUFF, RG;VOGT, PK
能够在对A和B亚群具有抗性的细胞中生长的禽肿瘤病毒可以被分配到两个新的亚群，C和D。C亚组的成员是病毒B77、7型和49型与肉瘤相关的病毒以及布拉格株劳斯肉瘤病毒的克隆纯化系。这些试剂在C O、CA、C B和C AB型鸡胚成纤维细胞培养物中具有相同的平板效率，但不包括C BC型鸡细胞。C亚群的肉瘤相关病毒干扰该亚群的肉瘤病毒，但不干扰A和B亚群的肉瘤病毒。相反，A亚群和B亚群的HCV相关病毒不能单独干扰或与C亚群的攻毒病毒联合干扰。中和试验和荧光抗体染色表明C亚群病毒的免疫相关性以及与A亚群和B亚群的抗原性差异。在C亚组肉瘤病毒中，只有B77在仓鼠中是致癌的。亚组D的成员是劳斯肉瘤病毒、卡尔齐尔伯相关病毒和50型罗氏相关病毒的施密特-鲁平和卡尔齐尔伯株的克隆衍生物。这些病毒在C O、C A、C B、C AB和C BC鸡胚成纤维细胞培养物中形成病灶。然而，在对亚组B耐药的所有细胞类型中，它们的平板接种效率显著降低。所有亚组D肉瘤病毒对新生仓鼠都具有高度致癌性。相关病毒能够干扰D亚群的肉瘤病毒，但这种干扰并不存在于所有遗传类型的鸡细胞中。D亚群相关病毒也干扰B亚群肉瘤病毒，B亚群肉瘤相关病毒诱导对D亚群的耐药性。亚组A或C与亚组D之间不发生干扰。亚群D的成员在免疫学上也是相关的，如中和和荧光抗体试验中交叉反应的高发生率和程度所示。似乎先前描述的亚组A和B主要代表西半球的禽类肿瘤病毒，而亚组C和D主要由在欧洲实验室中繁殖的病毒组成。
Avian tumor viruses capable of growing in cells resistant to the A and B subgroups can be assigned to two new subgroups, C and D. Members of subgroup C are virus B77, Rous-associated viruses types 7 and 49, and a clone-purified line of Prague strain Rous sarcoma virus. These agents plate with equal efficiency in types C O, C A, C B, and C AB chick embryo fibroblast cultures but are excluded from type C BC chicken cells. Rous-associated viruses of subgroup C interfere with sarcoma viruses of that subgroup but do not interfere with sarcoma viruses of subgroups A and B. Conversely, subgroup A and B Rous-associated viruses are unable to interfere alone or in combination with challenge viruses of subgroup C. Neutralization tests and fluorescent antibody staining indicate immunological relatedness of subgroup C viruses and antigenic distinctness from subgroups A and B. Of the subgroup C sarcoma viruses only B77 is oncogenic in hamsters. Members of subgroup D are cloned derivatives of the Schmidt-Ruppin and Carr Zilber strains of Rous sarcoma virus, Carr Zilber-associated virus, and Rous-associated virus type 50. These viruses form foci in C O, C A, C B, C AB, and C BC chick embryo fibroblast cultures. However, their plating efficiency is significantly reduced in all cell types resistant to subgroup B. All subgroup D sarcoma viruses are highly oncogenic for the newborn hamster. The associated viruses are capable of interfering with the sarcoma viruses of subgroup D, but this interference is not seen in all genetic types of chicken cells. The associated viruses of subgroup D also interfere with subgroup B sarcoma viruses, and subgroup B Rous-associated virus induces resistance to subgroup D. Interference does not take place between subgroups A or C and subgroup D. The members of subgroup D are also related immunologically, as indicated by the high incidence and degree of cross reactions in neutralization and fluorescent antibody tests. It appears that the previously described subgroups A and B represent mainly avian tumor viruses of the Western Hemisphere, whereas subgroups C and D are largely composed of viruses propagated in European laboratories.