First report of Echinococcus multilocularis in cats in Poland: a monitoring study in cats and dogs from a rural area and animal shelter in a highly endemic region
First report of Echinococcus multilocularis in cats in Poland: a monitoring study in cats and dogs from a rural area and animal shelter in a highly endemic region
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DOI：
10.1186/s13071-019-3573-x
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发表时间：
2019-06-24
影响因子：
3.2
通讯作者：
Cencek, Tomasz
中科院分区：
文献类型：
--
作者：
Karamon, Jacek;Sroka, Jacek;Cencek, Tomasz
泡状棘球绦虫病是由多房棘球绦虫幼虫引起的一种危险的人畜共患病。在其生命周期中，主要的最终宿主是红狐；然而，家养的食肉动物(狗和猫)也可以充当最终宿主。到目前为止，还没有关于波兰猫感染这种疾病的数据。本研究的目的是评估来自农村地区和动物收容所的猫和狗中多房球虫的流行率，该地区是红狐多房球虫的高发区。方法从波兰东南部一个高度流行的地区的农村地区(村庄和动物收容所)收集67只猫和268只狗的粪便样本。用套式聚合酶链式反应(多房棘球绦虫)、多重聚合酶链式反应(多房棘球绦虫、带绦虫)进行检测。和聚合酶链式反应[细粒棘球绦虫[E.graosus(s.l.)此外，对粪便进行显微镜检查(浮选)。结果4只猫(6.0%)和4只狗(1.5%)检出多房棘球绦虫。在猫和狗身上多房棘球绦虫的流行率方面，动物群体(收容所和主人)之间没有显著差异。带绦虫属10只猫(14.9%)(带绦虫和包虫病)和26只狗(9.7%)(弓形虫、毛滴虫、毛滴虫、豆形毛滴虫和绵羊毛滴虫)和4只猫(6.0%)和3只狗(1.1%)发现斜纹夜蛾。所有样本的聚合酶链式反应均为阴性。结合聚合酶链式反应和浮选结果，有29只猫(43.3%)和73只狗(27.2%)感染了蠕虫(分别为26.9%和11.9%)。通过对红狐的检查，确认了调查地区的高度流行状态：48.2%的受检红狐感染了多房埃希氏菌。结论据我们所知，本研究首次在波兰报道了猫中多房埃希氏菌的存在，并证实了在高度流行地区狗在这种感染中的作用。
BackgroundAlveolar echinococcosis is a dangerous zoonotic disease caused by larval forms of Echinococcus multilocularis. In its life-cycle, the principal definitive host is the red fox; however, domesticated carnivorous animals (dogs and cats) can also act as definitive hosts. Until now, there were no data concerning this infection in cats in Poland. The aim of this study was to estimate the prevalence of E. multilocularis in cats and dogs originating from rural areas and animal shelters in a region characterised by a high prevalence of E. multilocularis in red foxes.MethodsSamples of faeces were collected from 67 cats and 268 dogs from a rural area (villages and animal shelters) of a highly endemic region in southeastern Poland. Samples were examined using nested PCR (E. multilocularis), multiplex PCR (E. multilocularis, Taenia spp.) and PCR [E. granulosus (s.l.)]. Additionally, faeces were examined microscopically (flotation). Moreover, intestines from 110 red foxes shot in the investigated area were examined (sedimentation and counting technique).ResultsPositive PCR results for E. multilocularis were obtained in 4 cats (6.0%) and 4 dogs (1.5%). There were no significant differences between groups of animals (from a shelter and with an owner) concerning the prevalence of E. multilocularis in both cats and dogs. Taenia spp. were found in 10 cats (14.9%) (Taenia taeniaeformis and T. hydatigena) and 26 dogs (9.7%) (T. hydatigena, T. serialis, T. taeniaeformis, T. crassiceps, T. pisiformis and T. ovis) and Mesocestoides litteratus was found in 4 cats (6.0%) and 3 dogs (1.1%). All samples were negative for E. granulosus by PCR. Taking into consideration PCR and flotation results, 29 cats (43.3%) and 73 dogs (27.2%) were infected with helminths (26.9 and 11.9%, respectively, were infected with tapeworms). The highly endemic status of the investigated area was confirmed by examination of red foxes: 48.2% of examined red foxes were infected with E. multilocularis.ConclusionsTo the best of our knowledge, this study reports the presence of E. multilocularis in cats for the first time in Poland and confirms the role of dogs in this infection in highly endemic areas.