Complete Digital Pathology for Routine Histopathology Diagnosis in a Multicenter Hospital Network
Complete Digital Pathology for Routine Histopathology Diagnosis in a Multicenter Hospital Network
复制标题
多中心医院网络中常规组织病理学诊断的完整数字病理学
DOI：
10.5858/arpa.2018-0541-oa
复制
发表时间：
2020-02-01
影响因子：
4.6
通讯作者：
del Moral, Raimundo G.
中科院分区：
文献类型：
--
作者：
Antonio Retamero, Juan;Aneiros-Fernandez, Jose;del Moral, Raimundo G.
背景。-用于常规组织病理学诊断的完整数字病理学和全切片成像目前在全球少数实验室中使用。西班牙格拉纳达大学医院由4家医院组成，于2016年采用全数字病理学进行初步组织病理学诊断。描述格拉纳达大学医院在向全数字化诊断过渡时所采用的方法和由此产生的经验。使用Philips IntelliSite病理学解决方案（包括超快扫描仪和图像管理系统）将所有用于常规诊断的组织病理学载玻片以x40进行数字化。所有苏木精-伊红染色的制备物以及免疫组织化学和组织化学载玻片均数字化。现有的样本跟踪软件和图像管理系统被集成，以允许通过健康水平7协议进行数据交换。大约16万份标本已使用数字病理学进行初步诊断。这包括80多万张数字化载玻片。在实施阶段的扫描错误率低于1.5%，随后的工作流程优化使这一比率可以忽略不计。自实施以来，格拉纳达大学医院病理学家平均每年多签出21%的病例。数字病理学是初步组织病理学诊断的适当媒介。成功的数字化依赖于现有的样本跟踪和信息技术基础设施的整合。快速可靠的x40等效扫描是向全数字化工作流程过渡的关键。数字病理学提高了预分析和分析阶段的效率，并为计算病理学奠定了基础：使用计算机辅助工具来辅助诊断。
Context.-Complete digital pathology and whole slide imaging for routine histopathology diagnosis is currently in use in few laboratories worldwide. Granada University Hospitals, Spain, which comprises 4 hospitals, adopted full digital pathology for primary histopathology diagnosis in 2016.Objective.-To describe the methodology adopted and the resulting experience at Granada University Hospitals in transitioning to full digital diagnosis.Design.-All histopathology glass slides generated for routine diagnosis were digitized at x40 using the Philips IntelliSite Pathology Solution, which includes an ultrafast scanner and an image management system. All hematoxylin-eosin-stained preparations and immunohistochemistry and histochemistry slides were digitized. The existing sample-tracking software and image management system were integrated to allow data interchange through the Health Level 7 protocol.Results.-Circa 160 000 specimens have been signed out using digital pathology for primary diagnosis. This comprises more than 800 000 digitized glass slides. The scanning error rate during the implementation phase was below 1.5%, and subsequent workflow optimization rendered this rate negligible. Since implementation, Granada University Hospitals pathologists have signed out 21% more cases per year on average.Conclusions.-Digital pathology is an adequate medium for primary histopathology diagnosis. Successful digitization relies on existing sample tracking and integration of the information technology infrastructure. Rapid and reliable scanning at x40 equivalent was key to the transition to a fully digital workflow. Digital pathology resulted in efficiency gains in the preanalytical and analytical phases, and created the basis for computational pathology: the use of computer-assisted tools to aid diagnosis.