Differences in gene expression profiles and carcinogenesis pathways between colon and rectal cancer
Differences in gene expression profiles and carcinogenesis pathways between colon and rectal cancer
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DOI：
10.1111/j.1751-2980.2011.00551.x
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发表时间：
2012-01-01
影响因子：
3.5
通讯作者：
Qian, Jia Ming
中科院分区：
文献类型：
--
作者：
Li, Jing Nan;Zhao, Li;Qian, Jia Ming
目的：结肠癌在美国和欧洲比在中国更常见，原因尚不清楚。本研究的目的是探讨结肠癌和直肠癌基因表达谱和癌变途径的差异。方法：对12例结肠癌和12例直肠癌的原发肿瘤组织进行寡核苷酸芯片分析。所有样本均与临床特征严格匹配。生物信息学分析，如京都基因和基因组百科全书，被用于识别与结肠癌或直肠癌特别相关的基因和途径。结果：824个基因在结肠癌和直肠癌中存在差异表达。分析了信号网络中所有差异基因的相互作用。与结肠癌相比，直肠癌的基因差异表达和信号网中包含的基因更多。在结直肠癌差异表达基因中，S100P、Reg家族、ACTN1、CAMK2G和ACAT1的变化最为显著。参与细胞周期途径的基因存在于直肠癌和结肠癌中，但在直肠癌中更为重要。p53和代谢信号通路在结肠癌和直肠癌中存在显著差异。基因表达谱在结肠癌和直肠癌之间存在差异，代谢途径在直肠癌中更为重要。结论：直肠癌的发生可能比结肠癌复杂。一些基因可能成为区分这两种癌症的新生物标志物。
OBJECTIVE: Colon cancer is more common in the USA and Europe than that in China, for reasons that are unclear. The aim of this study was to investigate the differences in gene expression profiles and carcinogenesis pathways between colon and rectal cancer.METHODS: Expression profiling of primary tumor tissues from 12 colon and 12 rectal cancers was performed using oligonucleotide microarray analysis. All samples were strictly matched by clinical features. Bioinformatic analyses such as the Kyoto Encyclopedia of Genes and Genomes were used to identify genes and pathways specifically associated with colon or rectal cancers.RESULTS: A total of 824 genes were differentially expressed in colon and rectal cancers. All differential gene interactions in the Signal-Net were analyzed. More genes were differentially expressed and included in the Signal- Net for rectal than colon cancer. Of the genes differentially expressed between colon and rectal cancer, S100P, the Reg family, ACTN1, CAMK2G and ACAT1 were the most significantly altered. Genes involved in the cell cycle pathway were present in rectal and colon cancers, but were more important in rectal cancer. The p53 and metabolic signaling pathways were significantly different in colon and rectal cancers. Gene expression profiles differed between colon and rectal cancer, with metabolic pathways being more important in rectal cancer.CONCLUSION: The oncogenesis of rectal cancer may be more complex than that of colon cancer. Some genes could be new biomarkers for distinguishing between these two cancers.