Cyclic tensile stretch loaded on bovine chondrocytes causes depolymerization of hyaluronan - Involvement of reactive oxygen species
Cyclic tensile stretch loaded on bovine chondrocytes causes depolymerization of hyaluronan - Involvement of reactive oxygen species
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DOI：
10.1002/art.11305
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发表时间：
2003-11-01
影响因子：
--
通讯作者：
Hamanishi, C
中科院分区：
文献类型：
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作者：
Yamazaki, K;Fukuda, K;Hamanishi, C
目标。我们之前已经证明活性氧（ROS）参与软骨降解。透明质酸（HA），滑膜液中的主要大分子，它赋予粘度的大小减小，在关节炎患者中有报道。本研究的目的是确定由于软骨细胞承受机械变形而导致的HA分子量范围的改变，以及ROS在这一作用中的参与。活性氧是通过黄嘌呤氧化酶氧化次黄嘌呤产生的。采用真空操作装置加载循环拉伸拉伸。采用夹心酶结合法测定HA水平。采用水溶性四氮唑和化学发光探针分别测定了超氧化物歧化酶（SOD）活性和活性氧（ROS）。活性氧解聚透明质酸分子。循环拉伸拉伸使HA解聚并诱导ROS。SOD不仅能抑制ROS的诱导，还能抑制机械应力引起的HA解聚。活性氧在机械应力诱导的HA解聚中起重要作用。
Objective. We have previously demonstrated that reactive oxygen species (ROS) are involved in cartilage degradation. Decreased size of hyaluronan (HA), the major macromolecule in synovial fluid, to which it imparts viscosity, is reported in patients with arthritis. The purpose of this study was to determine the alteration in the molecular weight range of HA as a result of mechanical deformation loaded on the chondrocytes, as well as the involvement of ROS in this action.Methods. ROS were generated via the oxidation of hypoxanthine by xanthine oxidase. Cyclic tensile stretch was loaded using a vacuum-operated instrument. Levels of HA were measured using a sandwich enzyme-binding assay. Superoxide dismutase (SOD) activity and ROS were measured using water-soluble tetrazolium and a chemiluminescent probe, respectively.Results. ROS depolymerized HA molecules. Cyclic tensile stretch depolymerized HA and induced ROS. SOD inhibited not only ROS induction but also HA depolymerization caused by the mechanical stress.Conclusion. ROS play an important role in mechanical stress-induced HA depolymerization.