Chemodetection and Destruction of Host Urea Allows Helicobacter pylori to Locate the Epithelium.
Chemodetection and Destruction of Host Urea Allows Helicobacter pylori to Locate the Epithelium.
复制标题
宿主尿素的化学检测和破坏使幽门螺杆菌可以定位上皮。
DOI：
10.1016/j.chom.2015.07.002
复制
发表时间：
2015-08-12
影响因子：
30.3
通讯作者：
Amieva MR
中科院分区：
文献类型：
--
作者：
Huang JY;Sweeney EG;Sigal M;Zhang HC;Remington SJ;Cantrell MA;Kuo CJ;Guillemin K;Amieva MR
胃病原体幽门螺杆菌与胃粘膜密切相互作用，以避免杀微生物酸进入胃腔。幽门螺杆菌定位和定植胃上皮的线索尚未明确。我们发现从人类胃类器官产生的代谢物迅速吸引幽门螺杆菌。这种反应在很大程度上由细菌化学受体TlpB控制，而来自上皮的主要引诱剂是尿素。我们之前的结构分析表明，TlpB与尿素具有高亲和力。在这里，我们证明了这种紧密结合控制着高度敏感的反应，允许检测低至50纳摩尔的尿素浓度。对尿素的吸引需要幽门螺杆菌脲酶同时破坏信号。我们认为幽门螺杆菌已经进化出一种敏感的尿素化学检测和破坏系统，允许细菌动态和局部修改宿主环境以定位上皮。
The gastric pathogen Helicobacter pylori interacts intimately with the gastric mucosa to avoid the microbicidal acid in the stomach lumen. The cues H. pylori senses to locate and colonize the gastric epithelium have not been well defined. We show that metabolites emanating from human gastric organoids rapidly attract H. pylori. This response is largely controlled by the bacterial chemoreceptor TlpB, and the main attractant emanating from epithelia is urea. Our previous structural analyses show that TlpB binds urea with high affinity. Here we demonstrate that this tight binding controls highly sensitive responses, allowing detection of urea concentrations as low as 50 nanomolar. Attraction to urea requires that H. pylori urease simultaneously destroys the signal. We propose that H. pylori has evolved a sensitive urea chemodetection and destruction system that allows the bacterium to dynamically and locally modify the host environment to locate the epithelium.