Pseudomonas aeruginosa promotes Escherichia coli biofilm formation in nutrient-limited medium.
Pseudomonas aeruginosa promotes Escherichia coli biofilm formation in nutrient-limited medium.
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DOI：
10.1371/journal.pone.0107186
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Packman AI
中科院分区：
文献类型：
--
作者：
Culotti A;Packman AI
生物膜被认为是天然和工程水系统中病原体和共生肠道细菌（如大肠杆菌）的重要储存库。然而，调控大肠杆菌在水生生物膜中存活的过程尚未得到深入研究。我们研究了流体动力剪切和营养浓度对大肠杆菌在预先建立的铜绿假单胞菌生物膜上的定殖、大肠杆菌和铜绿假单胞菌生物膜的共接种以及铜绿假单胞菌在预先建立的大肠杆菌生物膜上的定殖的影响。在营养受限的R2A培养基中，当与铜绿假单胞菌共接种时，大肠杆菌在生物膜中占主导地位，并成功定植和过度生长预先建立的铜绿假单胞菌生物膜。在更丰富的培养基中，铜绿假单胞菌形成更大的簇，但大肠杆菌仍然广泛过度生长并定植在铜绿假单胞菌簇的内部。在单一培养中，大肠杆菌形成稀疏和不连续的生物膜。P. aeruginosa被引入这些生物膜后，大肠杆菌的生长显著增加，导致生物膜的定植模式与其他生物引入序列相似，即大肠杆菌过度生长P. aeruginosa并定植在P. aeruginosa簇的内部。这些结果表明，在营养匮乏的条件下，大肠杆菌不仅存在于水生生物膜中，而且在这些实验条件下，与铜绿假单胞菌的相互作用可以极大地促进大肠杆菌在生物膜中的生长。
Biofilms have been implicated as an important reservoir for pathogens and commensal enteric bacteria such as Escherichia coli in natural and engineered water systems. However, the processes that regulate the survival of E. coli in aquatic biofilms have not been thoroughly studied. We examined the effects of hydrodynamic shear and nutrient concentrations on E. coli colonization of pre-established Pseudomonas aeruginosa biofilms, co-inoculation of E. coli and P. aeruginosa biofilms, and P. aeruginosa colonization of pre-established E. coli biofilms. In nutritionally-limited R2A medium, E. coli dominated biofilms when co-inoculated with P. aeruginosa, and successfully colonized and overgrew pre-established P. aeruginosa biofilms. In more enriched media, P. aeruginosa formed larger clusters, but E. coli still extensively overgrew and colonized the interior of P. aeruginosa clusters. In mono-culture, E. coli formed sparse and discontinuous biofilms. After P. aeruginosa was introduced to these biofilms, E. coli growth increased substantially, resulting in patterns of biofilm colonization similar to those observed under other sequences of organism introduction, i.e., E. coli overgrew P. aeruginosa and colonized the interior of P. aeruginosa clusters. These results demonstrate that E. coli not only persists in aquatic biofilms under depleted nutritional conditions, but interactions with P. aeruginosa can greatly increase E. coli growth in biofilms under these experimental conditions.
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影响因子：
5.7
作者：
Lee J;Attila C;Cirillo SL;Cirillo JD;Wood TK
通讯作者：
Wood TK
影响因子：
4.4
作者：
LECHEVALLIER, MW;BABCOCK, TM;LEE, RG
通讯作者：
LEE, RG
DOI：
10.1099/13500872-140-8-2125
发表时间：
1994-08-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
ISAACS, H;CHAO, D;SAIER, MH
通讯作者：
SAIER, MH
影响因子：
2.8
作者：
Banning, N;Toze, S;Mee, BJ
通讯作者：
Mee, BJ
影响因子：
12.8
作者：
Fass, S;Dincher, ML;Block, JC
通讯作者：
Block, JC