LOCALIZATION OF THY-1 ANTIGEN IN RAT-BRAIN AND SPINAL-CORD BY IMMUNOFLUORESCENCE
LOCALIZATION OF THY-1 ANTIGEN IN RAT-BRAIN AND SPINAL-CORD BY IMMUNOFLUORESCENCE
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DOI：
10.1111/j.1471-4159.1978.tb06563.x
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发表时间：
1978-01-01
影响因子：
4.7
通讯作者：
HYDEN, H
中科院分区：
文献类型：
--
作者：
BARCLAY, AN;HYDEN, H
大鼠脑的Thy-1抗原是一种分子量为17，500的膜糖蛋白。使用抗纯化Thy-1和荧光素偶联的纯化羊F（ab“）2抗（兔IG[免疫球蛋白]G）抗体片段的兔抗血清，通过间接免疫荧光将其定位于脑和脊髓切片中。抗（Thy-1）血清的特异性通过定量间接放射性结合试验进行了测试，该试验特别适用于确定免疫组织化学研究中所用试剂的特异性。用纯化的Thy-1吸附抗-（Thy-1）血清作为免疫荧光实验的对照。在脑和脊髓的灰质中发现了强的特异性荧光，在白色物质中发现了少量的特异性荧光。所有神经细胞和髓鞘的细胞核缺乏荧光。一些大的神经元含有弱的胞质荧光，但大多数免疫荧光位于脑和脊髓的神经元。有迹象表明，Thy-1与突触结相关，因为它存在于突触肾小球中，并且在一些神经元周围呈颗粒状。不能排除与神经胶质膜的关联。
The Thy-1 antigen of rat brain is a membrane glycoprotein of MW 17,500. It was localized in sections of brain and spinal cord by indirect immunofluorescence using rabbit antisera raised against purified Thy-1 and fluorescein conjugated purified sheep F(ab'')2 anti-(rabbit Ig[immunoglobulin]G) antibody fragments. The specificity of the anti-(Thy-1) sera was tested by a quantitative indirect radioactive binding assay which is particularly useful for ascertaining the specificity of reagents used in immunohistochemical studies. Purified Thy-1 was used to absorb the anti-(Thy-1) sera for controls in the immunofluorescence experiments. Strong specific fluorescence was found throughout the gray matter of brain and spinal cord with lesser amounts in white matter. The nuclei of all neural cells and myelin lacked fluorescence. Some of the large neurons contained weak cytoplasmic fluorescence, but the majority of the immunofluorescence was located in the neuropil of the brain and spinal cord. There was an indication that Thy-1 was associated with synaptic knobs due to its presence in synaptic glomeruli and its granular appearance around some neurons. An association with glial membranes could not be excluded.