Topical pimecrolimus inhibits high-dose UVB irradiation-induced epidermal Langerhans cell migration, via regulation of TNF-α and E-cadherin.
Topical pimecrolimus inhibits high-dose UVB irradiation-induced epidermal Langerhans cell migration, via regulation of TNF-α and E-cadherin.
复制标题
局部吡美莫司通过调节 TNF-α 和 E-钙粘蛋白抑制高剂量 UVB 照射诱导的表皮朗格汉斯细胞迁移
DOI：
10.2147/dddt.s70790
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Luo D
中科院分区：
文献类型：
--
作者：
Yin Z;Xu J;Zhou B;Wu D;Xu Y;Zhang J;Luo D
外用吡美莫司可逆转高剂量紫外线（UV）B照射诱导的表皮CD 1a+朗格汉斯细胞减少，但其机制尚不清楚。本研究旨在探讨吡美莫司对高剂量UVB照射表皮郎格罕细胞的影响及其可能的机制。将40个人包皮组织分为四组：对照组、仅吡美莫司组、仅UVB组和UVB +吡美莫司组。培养所有组织，并将每个组织切成四块，对应四个时间点（0小时、18小时、24小时和48小时）。我们在每个时间点收集组织和培养基。流式细胞仪检测培养液中CD 1a+细胞的百分比。采用逆转录聚合酶链反应（PCR）和Western blot检测肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和E-cadherin的mRNA和蛋白表达。在18 h、24 h和48 h，UVB组和UVB +吡美莫司组培养液中的CD 1a+细胞明显多于对照组，而UVB +吡美莫司组的CD 1a+细胞少于UVB组。与对照组相比，UVB组和UVB +吡美莫司组在18 h、24 h和48 h的TNF-α表达（逆转录PCR和Western blot）明显升高，E-cadherin表达显著降低。UVB +吡美莫司组TNF-α明显低于单纯UVB组，E-cadherin明显高于单纯UVB组。局部应用吡美莫司可通过调节TNF-α和E-cadherin抑制大剂量UVB照射诱导的表皮朗格汉斯细胞迁移。
Topical pimecrolimus has been shown to reverse epidermal CD1a+ Langerhans cell reduction induced by high-dose ultraviolet (UV)B irradiation, but the mechanism is still unclear. This study aimed to investigate the possible mechanism of the effect of pimecrolimus on high-dose UVB-irradiated epidermal Langerhans cells. Forty human foreskin tissues were divided into four groups: control; pimecrolimus-only; UVB-only; and UVB + pimecrolimus. All tissues were cultured, and each tissue was cut into four pieces, corresponding to four time points (0 hours, 18 hours, 24 hours, and 48 hours). We collected the tissues and culture medium at each time point. The percentage of CD1a+ cells in medium was detected by flow cytometry. The tissues were detected for messenger (m)RNA and protein expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and E-cadherin, by reverse-transcription polymerase chain reaction (PCR) and Western blot. At 18 hours, 24 hours, and 48 hours, the CD1a+ cells in the culture medium of the UVB-only group and the UVB + pimecrolimus group were significantly more than in the control group, while the CD1a+ cells of the UVB + pimecrolimus group was less than of the UVB-only group. For both the UVB-only group and UVB + pimecrolimus group, TNF-α expression (by both reverse-transcription PCR and Western blot) of the tissues was clearly higher and E-cadherin expression was significantly lower compared with the control group, at 18 hours, 24 hours, and 48 hours. For the UVB + pimecrolimus group, TNF-α was clearly lower and E-cadherin was significantly higher compared with the UVB-only group. Topical pimecrolimus inhibited epidermal Langerhans cell migration induced by high-dose UVB irradiation, via regulation of TNF-α and E-cadherin.