RBM10 regulates alternative splicing
RBM10 regulates alternative splicing
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DOI：
10.1016/j.febslet.2014.01.052
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发表时间：
2014-03-18
期刊：
影响因子：
3.5
通讯作者：
Nakajima, Koichi
中科院分区：
文献类型：
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作者：
Inoue, Akira;Yamamoto, Naoki;Nakajima, Koichi
RBM 10，最初称为S1-1，是一种具有RNA加工蛋白特征结构域的核RNA结合蛋白。据报道，RBM 10构成剪接体复合物，并且RBM 5（RBM 10的密切同源物）调节阿尔茨海默病相关基因Fas和cFLIP的选择性剪接。在这项研究中，我们研究了RBM 10是否具有类似于RBM 5的剪接调节功能，并确定它确实调节Fas和Bcl-x基因的选择性剪接。RBM 10促进Fas前体mRNA的外显子跳跃以及Bcl-x前体mRNA中内部5 '剪接位点的选择。我们提出了一个RBM 10结合序列的目标外显子的5 '-剪接位点和RBM 10在选择性剪接的作用机制模型的共识。(C)2014年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
RBM10, originally called S1-1, is a nuclear RNA-binding protein with domains characteristic of RNA processing proteins. It has been reported that RBM10 constitutes spliceosome complexes and that RBM5, a close homologue of RBM10, regulates alternative splicing of apoptosis-related genes, Fas and cFLIP. In this study, we examined whether RBM10 has a regulatory function in splicing similar to RBM5, and determined that it indeed regulates alternative splicing of Fas and Bcl-x genes. RBM10 promotes exon skipping of Fas pre-mRNA as well as selection of an internal 5'-splice site in Bcl-x pre-mRNA. We propose a consensus RBM10-binding sequence at 5'-splice sites of target exons and a mechanistic model of RBM10 action in the alternative splicing. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.