DREF is required for EGFR signalling during Drosophila wing vein development
DREF is required for EGFR signalling during Drosophila wing vein development
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DOI：
10.1111/j.1365-2443.2004.00775.x
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发表时间：
2004-10
期刊：
影响因子：
2.1
通讯作者：
H. Yoshida;E. Kwon;F. Hirose;Kyoko Otsuki;Mikihiro Yamada;M. Yamaguchi
中科院分区：
文献类型：
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作者：
H. Yoshida;E. Kwon;F. Hirose;Kyoko Otsuki;Mikihiro Yamada;M. Yamaguchi
DNA复制相关元件结合因子（DREF）被认为参与果蝇中DNA复制和增殖相关基因的调控。最近，通过搜索果蝇基因组数据库，我们还发现了许多具有其他功能的基因的5′-侧翼区的DRE样序列。此外，用抗DREF单克隆抗体对多线染色体进行免疫染色显示，DREF可以与多线染色体的一百个区域结合，这表明在体内调节多个基因和多个角色。当我们在果蝇中使用GAL 4 ‐UAS靶向表达系统在翅盘中过表达DREF蛋白或DREF基因的反向重复RNA时，结果分别是静脉宽度增加和静脉损失。随着DREF的过表达，果蝇MAPK同源物Rolled被异位激活。此外，将D-raf基因剂量减少一半抑制了这种DREF诱导的宽度增加的静脉表型。此外，当通过将DREF基因的双链RNA引入S2细胞来减少DREF转录物时，D-raf基因启动子活性降低至4%。这些数据表明，DREF是参与调控静脉形成通过激活表皮生长因子受体信号在果蝇翅成虫盘。
The DNA replication‐related element binding factor (DREF) has been suggested as being involved in regulation of DNA replication‐ and proliferation‐related genes in Drosophila. Recently, by searching the Drosophila genome database, we also found DRE‐like sequences in the 5′‐flanking regions of many genes with other functions. In addition, immunostaining of polytene chromosomes with an anti‐DREF monoclonal antibody revealed that DREF can bind to a hundred regions of polytene chromosomes, suggesting regulation of multiple genes and multiple roles in vivo. When we over‐expressed DREF protein or inverted repeat RNA of the DREF gene in wing imaginal discs using the GAL4‐UAS targeted expression system in Drosophila, the results were veins of increased width and a loss of veins, respectively. With DREF over‐expression, Rolled, a Drosophila MAPK homologue, was ectopically activated. Furthermore, half reduction of the D‐raf gene dose suppressed this DREF‐induced vein of increased width phenotype. In addition, when DREF transcripts were reduced by introducing double‐stranded RNA of the DREF gene into S2 cells, the D‐raf gene promoter activity was diminished to 4%. These data indicate that DREF is involved in regulation of vein formation through the activation of EGFR signalling in the Drosophila wing imaginal discs.