Annotation transfer between genomes: Protein-protein interologs and protein-DNA regulogs
Annotation transfer between genomes: Protein-protein interologs and protein-DNA regulogs
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DOI：
10.1101/gr.1774904
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发表时间：
2004-06-01
期刊：
影响因子：
7
通讯作者：
Gerstein, M
中科院分区：
文献类型：
--
作者：
Yu, HY;Luscombe, NM;Gerstein, M
蛋白质主要通过相互作用发挥作用，特别是与DNA和其他蛋白质。虽然一些大规模的相互作用网络现在可用于一些模式生物，但它们的实验生成仍然很困难。因此，interlog映射-使用比较基因组学将相互作用注释从一个生物体转移到另一个生物体-具有重要价值。在这里，我们定量评估的程度interlogs可以可靠地转移物种之间作为相应的相互作用的蛋白质的序列相似性的函数。利用来自酿酒酵母、秀丽隐杆线虫、黑腹果蝇和幽门螺杆菌的相互作用信息，我们发现当一对蛋白质具有>80%的联合序列同一性或联合E值时，蛋白质-蛋白质相互作用可以转移。
Proteins function mainly through interactions, especially with DNA and other proteins. While some large-scale interaction networks are now available for a number of model organisms, their experimental generation remains difficult. Consequently, interolog mapping-the transfer of interaction annotation from one organism to another using comparative genomics-is of significant value. Here we quantitatively assess the degree to which interologs can be reliably transferred between species as a function of the sequence similarity of the corresponding interacting proteins. Using interaction information from Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, and Helicobacter pylori, we find that protein-protein interactions can be transferred when a pair of proteins has a joint sequence identity >80% or a joint E-value