Serum uncouples elevation of cyclic adenosine monophosphate concentration from cyclic adenosine monophosphate dependent morphological changes exhibited by cultured pituicytes.
Serum uncouples elevation of cyclic adenosine monophosphate concentration from cyclic adenosine monophosphate dependent morphological changes exhibited by cultured pituicytes.
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血清将环单磷酸腺苷浓度的升高与培养的垂体细胞表现出的环单磷酸腺苷依赖性形态变化解耦。
DOI：
10.1016/s0304-3940(97)00248-6
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发表时间：
1997
影响因子：
2.5
通讯作者：
Cobbett,P
中科院分区：
文献类型：
--
作者：
Ramsell,KD;Cobbett,P
培养的垂体细胞（神经垂体星形胶质细胞）在HEPES平衡盐溶液（HBSS）中培养（90分钟）时通常为扁平无定形细胞，但在添加forskolin的HBSS中培养时变为星形细胞。血清（0.5% vol/vol）可减弱这种星形过程。本文描述的实验旨在确定血清是否通过调节细胞内环磷酸腺苷（cAMP）浓度或其他机制来减弱星形。结果表明，百日咳毒素（100 ng/ml）不影响血清对福斯克林诱导的星形的影响，血清对磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤（IBMX; 100μ m）诱导的星形也有抑制作用。此外，血清可抑制膜透性cAMP类似物8-溴cAMP （150 μM）诱导的星形。这些结果表明，虽然细胞内cAMP浓度的增加是垂体星形形成的必要条件，但细胞内cAMP浓度的增加可能与星形形成脱钩。
Cultured pituicytes (neurohypophysial astrocytes) are normally flat amorphous cells when incubated (90 min) in a HEPES balanced salt solution (HBSS) but become stellate when incubated in HBSS supplemented with forskolin. This stellation process is attenuated by serum (0.5% vol/vol). The experiments described here were designed to determine whether serum attenuates stellation by modulation of the intracellular cyclic adenosine monophosphate (cAMP) concentration or some other mechanism. It was observed that the effect of serum on forskolin-induced stellation was not affected by pertussis toxin (100 ng/ml) and that serum also inhibited stellation induced by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX; 100μM). Further, serum inhibited stellation induced by the membrane permeable cAMP analog 8-bromo cAMP (150 μM). These results indicate that although an increase of intracellular cAMP concentration is necessary for pituicyte stellation, an increase of intracellular cAMP concentration may be decoupled from stellation.