Analysis of milk gland structure and function in Glossina morsitans: milk protein production, symbiont populations and fecundity.
Analysis of milk gland structure and function in Glossina morsitans: milk protein production, symbiont populations and fecundity.
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DOI：
10.1016/j.jinsphys.2008.06.008
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发表时间：
2008-08
影响因子：
2.2
通讯作者：
Aksoy, Serap
中科院分区：
文献类型：
--
作者：
Attardo, Geoffrey M.;Lohs, Claudia;Heddi, Abdelaziz;Alam, Uzma H.;Yildirim, Suleyman;Aksoy, Serap
采采蝇生殖周期的一个关键过程是将必需营养物质和细菌共生体从母亲转移到子宫内的后代。介导这种转移的组织是乳腺。这项工作重点研究两种乳蛋白（乳腺蛋白 (GmmMGP) 和转铁蛋白 (GmmTsf)）和采采蝇内共生体（Sodalis 和 Wigglesworthia）在乳腺生理学背景下的定位和功能。荧光原位杂交（FISH）和免疫组织化学分析证实乳腺分泌细胞合成并分泌乳腺蛋白和转铁蛋白。双链 RNA (dsRNA) 介导的 RNA 干扰敲低 gmmmgp 会导致采采蝇繁殖力降低，这证明了其在幼虫营养和发育中的功能重要性。乳腺切片的细菌物种特异性原位杂交揭示了乳腺腔内存在大量 Sodalis 和 Wigglesworthia。 Sodalis 也位于分泌细胞的细胞质内。在管腔内，Wigglesworthia 靠近通向乳腺分泌细胞的乳汁储存库的通道。我们讨论了乳腺在幼虫营养和共生细菌向发育中的后代传播中的重要性。
A key process in the tsetse reproductive cycle is the transfer of essential nutrients and bacterial symbionts from mother to intrauterine offspring. The tissue mediating this transfer is the milk gland. This work focuses upon the localization and function of two milk proteins (milk gland protein (GmmMGP) and transferrin (GmmTsf)) and the tsetse endosymbionts (Sodalis and Wigglesworthia), in the context of milk gland physiology. Fluorescent in situ hybridization (FISH) and immunohistochemical analysis confirm that the milk gland secretory cells synthesize and secrete milk gland protein and transferrin. Knockdown of gmmmgp by double stranded RNA (dsRNA) mediated RNA interference results in reduction of tsetse fecundity, demonstrating its functional importance in larval nutrition and development. Bacterial species-specific in situ hybridizations of milk gland sections reveal large numbers of Sodalis and Wigglesworthia within the lumen of the milk gland. Sodalis is also localized within the cytoplasm of the secretory cells. Within the lumen, Wigglesworthia localize close to the channels leading to the milk storage reservoir of the milk gland secretory cells. We discuss the significance of the milk gland in larval nutrition and in transmission of symbiotic bacteria to developing offspring.
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影响因子：
2.6
作者：
Cheng, Q;Aksoy, S
通讯作者：
Aksoy, S
DOI：
10.1016/0010-406x(69)91764-2
发表时间：
1969-01-01
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY
影响因子：
--
作者：
CMELIK, SHW;BURSELL, E;SLACK, E
通讯作者：
SLACK, E
影响因子：
2.6
作者：
MA, WC;DENLINGER, DL;SMITH, DS
通讯作者：
SMITH, DS
DOI：
10.1111/j.1749-6632.1975.tb35097.x
发表时间：
1975-01-01
影响因子：
5.2
作者：
DENLINGER, DL;MA, WC
通讯作者：
MA, WC
影响因子：
2.2
作者：
DENLINGER, DL;MA, WC
通讯作者：
MA, WC