Fc-gamma receptors are not involved in cartilage damage during experimental osteoarthritis.
Fc-gamma receptors are not involved in cartilage damage during experimental osteoarthritis.
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Fc-γ受体不参与实验性骨关节炎期间的软骨损伤
DOI：
10.1016/j.joca.2015.02.019
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发表时间：
2015
影响因子：
7
通讯作者：
Schett G
中科院分区：
文献类型：
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作者：
Stock M;Distler A;Distler J;Beyer C;Ruiz-Heiland G;Ipseiz N;Seeling M;Krönke G;Nimmerjahn F;Schett G
已证实，Fc γ受体（FcγRs）在实验性关节炎期间的软骨降解中起关键作用。尽管它们对软骨降解的大部分作用归因于它们在免疫球蛋白存在下促进炎症的潜力，但激活Fcγ R促进抗原诱导性关节炎（AIA）中的软骨退化，与炎症水平无关。方法采用RT-PCR和流式细胞术检测小鼠软骨组织和软骨细胞中Fc γR的表达。在WT小鼠和缺乏激活性（Fc受体γ链缺陷）或抑制性（Fcγ RIIB缺陷）Fcγ R的动物中，通过内侧半月板（DMM）去稳定诱导实验性OA。根据OARSI建议，通过评估蛋白聚糖损失和结构损伤，在术后8周对软骨损伤进行组织学研究。骨赘的大小进行了测量，以调查骨turn.ResultsExpression分析的变化显示，所有四种类型的小鼠Fcγ R在小鼠软骨细胞和软骨组织的新生儿和8周龄的小鼠显着水平。令人惊讶的是，然而，无论是激活或抑制FcγRs消融不影响软骨损伤或骨转换DMM诱导的OA在mice.ConclusionWhile FcγRs似乎有一个至关重要的作用，在软骨降解炎症性关节炎我们的数据表明，FcγRs不影响软骨破坏实验OA。这表明一定的炎症阈值是关节炎中Fcγ R诱导的软骨破坏的先决条件。
ObjectiveFc-gamma receptors (FcγRs) have been shown to play a crucial role in cartilage degradation during experimental arthritis. Although most of their effect on cartilage degradation has been attributed to their potential to promote inflammation in the presence of immunoglobulins, activating FcγRs promote cartilage degeneration in antigen-induced arthritis (AIA) independently of the level of inflammation. This prompted us to investigate, whether FcγRs may also play a role in osteoarthritis (OA)-related cartilage degradation.MethodsFcγR expression was measured by RT-PCR and FACS in murine cartilage tissue and chondrocytes. Experimental OA was induced by destabilisation of the medial meniscus (DMM) in WT mice and animals lacking either activating (Fc receptor γ-chain-deficient) or inhibitory (FcγRIIB-deficient) FcγRs. Cartilage damage was investigated histologically 8 weeks post-surgery by assessing proteoglycan loss and structural damage according to OARSI recommendations. Osteophyte size was measured to investigate alterations in bone turnover.ResultsExpression analyses revealed significant levels for all four types of murine FcγRs in mouse chondrocytes and cartilage tissue from newborn and 8-week-old mice. Surprisingly, yet, ablation of either activating or inhibitory FcγRs did not affect cartilage damage or bone turnover during DMM-induced OA in mice.ConclusionWhile FcγRs appear to have a crucial role in cartilage degradation during inflammatory arthritis our data indicate that FcγRs do not influence cartilage destruction in experimental OA. This indicates that a certain threshold of inflammation is a prerequisite for FcγR-induced cartilage destruction in arthritis.
影响因子：
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作者：
van Lent, Peter L. E. M.;Blom, Arjen B.;van den Berg, Wim B.
通讯作者：
van den Berg, Wim B.