Salidroside stimulated glucose uptake in skeletal muscle cells by activating AMP-activated protein kinase
Salidroside stimulated glucose uptake in skeletal muscle cells by activating AMP-activated protein kinase
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DOI：
10.1016/j.ejphar.2008.04.036
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发表时间：
2008-07-07
影响因子：
5
通讯作者：
Zhang, Le
中科院分区：
文献类型：
--
作者：
Li, Han-Bing;Ge, Ya-kun;Zhang, Le
本文报道了红景天活性成分之一红景天苷对骨骼肌细胞代谢的影响。红景天苷剂量依赖性地刺激分化的L 6大鼠成肌细胞的葡萄糖摄取。通过用化合物C预处理细胞的AMP活化蛋白激酶（AMPK）抑制剂有效地降低了红景天苷刺激的葡萄糖摄取，而渥曼青霉素对磷脂酰肌醇3-激酶（PI 3 K）的抑制对红景天苷介导的葡萄糖转运活化没有表现出显著的抑制作用。Western blotting分析显示红景天苷增加了AMPK和乙酰辅酶A羧化酶（ACC）的磷酸化水平。此外，红景天苷增强胰岛素介导的Akt活化和葡萄糖摄取，并且这种增强可以被化合物C特异性抑制。综上所述，AMPK激活参与了红景天苷对葡萄糖转运激活和胰岛素敏感性的影响。红景天苷可作为潜在的抗糖尿病药物进一步开发。(C)2008 Elsevier B. V.保留所有权利。
in the present study, we reported the metabolic effects of salidroside, one of the active components of Rhodiola Rosea, on skeletal muscle cells. Salidroside dose-dependently stimulated glucose uptake in differentiated L6 rat myoblast cells. Inhibitor of AMP-activated protein kinase (AMPK) by pretreating the cells with compound C potently reduced salidroside-stimulated glucose uptake, while inhibition of phosphatidylinositol 3-kinase (PI3K) by wortmannin exhibited no significant inhibitory effect on salidroside-mediated glucose transport activation. Western blotting analyses revealed that salidroside increased the phosphorylation level of AMPK and acetyl-CoA carboxylase (ACC). In addition, salidroside enhanced insulin-mediated Akt activation and glucose uptake, and such enhancement can be specifically inhibited by compound C. In summary, AMPK activation was involved in the effects of salidroside on glucose transport activation and insulin sensitivity. Salidroside can be further developed as potential compound for the anti-diabetic therapy. (C) 2008 Elsevier B.V. All rights reserved.