IL-4 down-regulates IL-1 and TNF gene expression in human monocytes.
IL-4 down-regulates IL-1 and TNF gene expression in human monocytes.
复制标题
DOI：
10.4049/jimmunol.142.11.3857
复制
发表时间：
1989-06
影响因子：
4.4
通讯作者：
R. Essner;K. Rhoades;W. McBride;D. Morton;J. Economou
中科院分区：
文献类型：
--
作者：
R. Essner;K. Rhoades;W. McBride;D. Morton;J. Economou
本文观察了IL-4对人外周血单核细胞（PBM）IL-1和TNF基因表达的影响。高度纯化的PBM在IL-4存在下培养24至96小时既不产生IL-1也不产生TNF蛋白。RNA杂交研究表明，IL-4不诱导IL-1或TNF的转录。然而，PBM与IL-4预孵育确实以剂量和时间相关的方式抑制LPS诱导的IL-1和TNF的产生。最大抑制发生在与200 U/ml IL-4孵育96 h后。RNA杂交研究表明，IL-4抑制IL-1 α，IL-1 β和TNF mRNA的表达。这些结果表明IL-4通过下调基因表达调节单核细胞产生TNF和IL-1。IL-4的这种独特性质在免疫应答的调节中可能是重要的。
The effects of IL-4 on IL-1 and TNF gene expression in human peripheral monocytes (PBM) were examined. Highly purified PBM cultured for 24 to 96 h in the presence of IL-4 produce neither IL-1 nor TNF protein. RNA hybridization studies demonstrated that IL-4 does not induce transcription of IL-1 or TNF. Preincubation of PBM with IL-4 did, however, inhibit LPS-induced IL-1 and TNF production in a dose- and time-related fashion. Maximal inhibition occurred after 96 h of incubation with 200 U/ml IL-4. RNA hybridization studies demonstrated that IL-4 suppressed the expression of IL-1 alpha, IL-1 beta, and TNF mRNA. These results suggest IL-4 modulates monocyte production of TNF and IL-1 by down-regulation of gene expression. This unique property of IL-4 may be important in the regulation of the immune response.