The wild rat as sentinel animal in the environmental risk assessment of asbestos pollution: A pilot study
The wild rat as sentinel animal in the environmental risk assessment of asbestos pollution: A pilot study
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DOI：
10.1016/j.scitotenv.2014.01.108
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发表时间：
2014-05-01
影响因子：
9.8
通讯作者：
Belluso, Elena
中科院分区：
文献类型：
--
作者：
Ardizzone, Michele;Vizio, Carlotta;Belluso, Elena
石棉在包括意大利在内的许多国家都被禁止使用。然而，暴露源可能仍然存在，这是由于原位石棉或过去对含石棉废物的处置。在过去高环境暴露的城市地区，如Casale Monferrato，哨点动物的肺纤维负荷可能有助于确定这些来源。进行了一项初步研究，以评估在野生大鼠中测定其可行性，野生大鼠是以前从未用于环境肺石棉纤维负荷研究的合适哨点物种。在虫害防治活动的框架内，从Casale Monferrato市区3个地点捕获了11只成年鼠，从另一个未暴露的城镇捕获了3只对照鼠。此外，从参与育种计划和对大鼠进行实验研究的实验室获得了3个阳性和3个阴性对照肺样本。用扫描电镜和能量色散光谱法测定组织纤维浓度。在Casale Monferrato大鼠的肺中发现了石棉（温石棉和青石棉），但在对照大鼠和阴性对照肺样本中未发现石棉。阳性对照肺标本中发现高浓度石棉绿铜矿。测量野生大鼠的肺纤维负荷已被证明是可行的：不仅可以检测到石棉纤维，而且可以定性和定量地表征石棉纤维。试点研究为使用野生老鼠作为Casale Monferrato土壤污染水平的哨兵提供了基本原理，以确定以前未被识别的石棉源可能存在的区域。(C) 2014 Elsevier B.V.版权所有
Asbestos has been banned in many countries, including Italy. However, sources of exposure may still exist, due to asbestos in-situ or past disposal of asbestos-containing waste. In an urban area with past high environmental exposure, like Casale Monferrato, the lung fiber burden in sentinel animals may be useful to identify such sources. A pilot study was conducted to assess the feasibility of its determination in wild rats, a suitable sentinel species never used before for environmental lung asbestos fiber burden studies.Within the framework of pest control campaigns, 11 adult animals from 3 sites in the urban area of Casale Monferrato and 3 control rats from a different, unexposed town were captured. Further, 3 positive and 3 negative control lung samples were obtained from laboratories involved in breeding programs and conducting experimental studies on rats. Tissue fiber concentration was measured by scanning electron microscopy with energy dispersive spectrometry.Asbestos (chlysotile and crocidolite) was identified in the lungs from rats from Casale Monferrato, but not in control rats and in negative control lung samples. Asbestos grunerite at high concentration was found in positive control lung samples.Measurement of the lung fiber burden in wild rats has proved feasible: it was possible not only to detect, but also to characterize asbestos fibers both qualitatively and quantitatively. The pilot study provides the rationale for using wild rats as sentinels of the soil contamination level in Casale Monferrato, to identify areas with the possible presence of previously unrecognized asbestos sources. (C) 2014 Elsevier B.V. All rights reserved.