Chronic stress affects the expression of brain-derived neurotrophic factor in rat salivary glands
Chronic stress affects the expression of brain-derived neurotrophic factor in rat salivary glands
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DOI：
10.3109/10253890902875167
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发表时间：
2010-01
期刊：
影响因子：
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通讯作者：
J. Saruta;T. Lee;M. Shirasu;Takeshi Takahashi;Chikatoshi Sato;S. Sato;K. Tsukinoki
中科院分区：
文献类型：
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作者：
J. Saruta;T. Lee;M. Shirasu;Takeshi Takahashi;Chikatoshi Sato;S. Sato;K. Tsukinoki
血浆脑源性神经营养因子(BDNF)水平与多种神经疾病有关。以前，我们曾报道过BDNF是由唾液腺在急性制动应激下产生的。此外，唾液腺是应激时血浆BDNF的来源；然而，在慢性应激条件下唾液腺表达BDNF之间的联系尚不清楚。在目前的研究中，我们调查了慢性应激时血浆BDNF水平是否依赖于唾液腺。雄性大鼠暴露于慢性束缚应激(每天12h，持续22d)后，颌下腺组织中可检测到脑源性神经营养因子基因和蛋白的表达。慢性应激显著增加双侧涎腺切除大鼠血浆BDNF浓度、促肾上腺皮质激素和皮质酮水平，但在慢性应激下无明显变化。由于慢性应激增加了唾液腺切除大鼠模型的血浆BDNF水平，血浆BDNF水平不依赖于唾液腺的BDNF水平。虽然在我们先前的研究中，唾液腺是急性应激条件下血浆BDNF的来源，但在慢性应激条件下，唾液腺中BDNF表达的增加似乎并不是循环BDNF水平升高的重要原因。血浆脑源性神经营养因子水平的升高可能在应激状态下的动态平衡中起重要作用。
Plasma brain-derived neurotrophic factor (BDNF) levels are associated with several neural disorders. Previously, we reported that BDNF is produced from salivary glands under acute immobilization stress. Additionally, salivary glands are the origin of plasma BDNF during stress; however, the association between the expression of BDNF by the salivary glands under chronic stress conditions is not known. In the present study, we investigated whether plasma BDNF levels in chronic stress depend on the salivary glands. Expression of BDNF mRNA and protein were identified in the submandibular glands when male rats were exposed to chronic restraint stress (12 h daily for 22 days). Chronic stress significantly increased plasma BDNF concentration, as well as adrenocorticotropic hormone and corticosterone levels, but was not altered under chronic stress in bilaterally sialoadenectomized rats. Since chronic stress increases plasma BDNF levels in the sialoadenectomized rat model, the plasma BDNF level was not dependent on BDNF from the salivary glands. Although the salivary glands were the source of plasma BDNF in acute stress conditions in our previous study, it seems that that the increased BDNF expression in the salivary glands in chronic stress does not contribute importantly to the increased circulating BDNF level. The increased plasma BDNF levels may play important roles in homeostasis under stress conditions.