Genotyping of Cytomegalovirus from Symptomatic Infected Neonates in Iraq
Genotyping of Cytomegalovirus from Symptomatic Infected Neonates in Iraq
复制标题
DOI：
10.4269/ajtmh.18-0152
复制
发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Wickes, Brian L.
中科院分区：
文献类型：
--
作者：
Alwan, Sevan N.;Shamran, Haidar A.;Wickes, Brian L.
在所有其他病毒中，人类巨细胞病毒（HCMV）是全球先天性感染最常见的原因。 HCMV 菌株变异可以预测先天性 HCMV 疾病的严重程度或结果。先前的研究已将特定基因型与特定后遗症或更严重的疾病联系起来，但结果是矛盾的。之前没有针对伊拉克 HCMV 基因型的研究。因此，本研究旨在对从有症状的先天/围产期感染新生儿中分离出的 HCMV 进行分子检测和基因分型。这项前瞻性研究包含 24 份来自患有先天性/围产期感染的有症状新生儿的血清样本。从这些血清样本中提取病毒DNA；使用巢式聚合酶链式反应来扩增 HCMV gB (UL55) 基因。第二轮扩增的聚合酶链式反应产物进行直接Sanger测序。 Bioedit 和 MEGA5 软件（EMBL-EBI，Hinxton，Cambridgeshire，UK）用于比对和构建系统发育树。 24 个样本中有 23 个样本检测到人巨细胞病毒 DNA（95.8%）。根据系统发育分析，鉴定出该病毒有3种基因型； gB1、gB2 和 gB3 基因型。然而，没有检测到gB4基因型。人类巨细胞病毒gB3是最常见的基因型：在有症状的感染婴儿中，24例中有14例（58.33%），其次是gB1（6/24；25%）和gB2（4/24；16.67%）。仅在 1 例病例中检测到 gB3/gB1 混合 HCMV 感染。人类巨细胞病毒 gB3 是有症状的先天/围产期 HCMV 感染新生儿中最主要的基因型。未发现 B3 基因型与特定临床表现之间存在关联。黄疸是有症状感染的新生儿中最常见的临床特征，其次是肝脾肿大。
Among all other viruses, human cytomegalovirus (HCMV) is the most frequent cause of congenital infection worldwide. Strain variation in HCMV may predict severity or outcome of congenital HCMV disease. Previous studies have associated a particular genotype with specific sequelae or more severe illness, but the results were contradictory. There are no previous studies addressing the genotype of HCMV in Iraq. Therefore, the present study is aimed at molecular detection and genotyping of HCMV isolated from symptomatic congenitally/perinatally infected neonates. This prospective study comprised 24 serum samples from symptomatic neonates with congenital/perinatal infection. Viral DNA was extracted from these serum samples; nested polymerase chain reaction was used to amplify the HCMV gB (UL55) gene. Polymerase chain reaction products of the second round of amplification were subjected to direct Sanger sequencing. Bioedit and MEGA5 software (EMBL-EBI, Hinxton, Cambridgeshire, UK) were used for alignment and construction of a phylogenetic tree. Human cytomegalovirus DNA was detected in 23 of 24 samples (95.8%). According to the phylogenetic analysis, three genotypes of the virus were identified; gB1, gB2, and gB3 genotypes. However, the gB4 genotype was not detected. Human cytomegalovirus gB3 was the most frequent genotype: 14 of 24 (58.33%) among symptomatic infected infants, followed by gB1 (6/24; 25%) and gB2 (4/24; 16.67%). A mixed HCMV infection with gB3/gB1 was detected in only one case. Human cytomegalovirus gB3 was the most predominant genotype among symptomatic congenitally/perinatally HCMV-infected neonates. No association was found between B3 genotype and specific clinical presentation. Jaundice was the most common clinical feature among symptomatically infected neonates, followed by hepatosplenomegaly.