Microfluidic immunoassays as rapid saliva-based clinical diagnostics
Microfluidic immunoassays as rapid saliva-based clinical diagnostics
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DOI：
10.1073/pnas.0607254104
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发表时间：
2007-03-27
影响因子：
11.1
通讯作者：
Singh, Anup K.
中科院分区：
文献类型：
--
作者：
Herr, Amy E.;Hatch, Anson V.;Singh, Anup K.
目前，护理点（POC）诊断通常提供健康状态的二进制指示（例如，家用妊娠测试条）。在预期使用诊断评估复杂的疾病变得普遍，先进的生物测定能够定量测量疾病的生物标志物的发展是必要的。将新的生物测定成功地转化为临床环境需要能够监测疾病的发作和进展。在这里，我们报告了一种临床POC诊断方法，该方法可以通过使用设计用于在紧凑型分析仪器中操作的整体式一次性检测盒来快速定量人类唾液中的口腔疾病生物标志物。我们的微流控方法通过将样品预处理（过滤、富集、混合）与电泳免疫测定相结合，以快速测量最低程度预处理的唾液样品中的分析物浓度，从而促进了免提唾液分析。使用20 μ l的唾液，我们证明了快速（< 10分钟）测量的胶原裂解酶基质金属蛋白酶-8（MMP-8）的唾液从健康和牙周疾病的受试者。除了牙周病的生理学可测量指标外，还使用唾液MMP-8的常规测量来验证本原理验证研究中描述的微流体测定。所证明的基于POC的诊断适用于快速、可靠地测量生物流体中的蛋白质类疾病生物标志物。
At present, point-of-care (POC) diagnostics typically provide a binary indication of health status (e.g., home pregnancy test strip). Before anticipatory use of diagnostics for assessment of complex diseases becomes widespread, development of sophisticated bioassays capable of quantitatively measuring disease biomarkers is necessary. Successful translation of new bioassays into clinical settings demands the ability to monitor both the onset and progression of disease. Here we report on a clinical POC diagnostic that enables rapid quantitation of an oral disease biomarker in human saliva by using a monolithic disposable cartridge designed to operate in a compact analytical instrument. Our microfluidic method facilitates hands-free saliva analysis by integrating sample pretreatment (filtering, enrichment, mixing) with electrophoretic immunoassays to quickly measure analyte concentrations in minimally pretreated saliva samples. Using 20 mu l of saliva, we demonstrate rapid (< 10 min) measurement of the collagen-cleaving enzyme matrix metalloproteinase-8 (MMP-8) in saliva from healthy and periodontally diseased subjects. In addition to physiologically measurable indicators of periodontal disease, conventional measurements of salivary MMP-8 were used to validate the microfluidic assays described in this proof-of-principle study. The based POC diagnostic demonstrated is applicable to rapid, reliable measurement of proteinaceous disease biomarkers in biological fluids.