Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma.
Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma.
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综合 miRNA 分析和生物信息学分析揭示了胃腺癌中潜在的致病 miRNA
DOI：
10.18632/oncotarget.5419
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发表时间：
2015-10-20
期刊：
影响因子：
--
通讯作者：
Meltzer SJ
中科院分区：
文献类型：
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作者：
Zhang X;Peng Y;Jin Z;Huang W;Cheng Y;Liu Y;Feng X;Yang M;Huang Y;Zhao Z;Wang L;Wei Y;Fan X;Zheng D;Meltzer SJ
胃癌（GC）是整个中国和全球与癌症相关死亡的主要原因之一。 MicroRNA（miRNA）的发现为开发诊断生物标志物和GC中有效的治疗靶标提供了新的机会。通过对良性和恶性胃皮细胞系进行微阵列分析（HFE145，NCI-N87，MKN28，RF1，Kato III和RF48），发现了16个明显失调的miRNA。其中11通过实时QRT-PCR验证。基于Mirwalk在线数据库扫描，确定了16个miRNA的703个潜在的mRNA靶标。生物信息学分析表明，这些失调的miRNA及其预测靶标主要参与肿瘤发病机理，MAPK信号传导和凋亡。最后，miRNA-Gene网络分析将miRNA-125b鉴定为GC发育中的关键miRNA。综上所述，这些结果形成了与胃肿瘤发生有关的差异表达的miRNA的全面表达和功能谱。该概况可以作为GC患者生物标志物和治疗靶标识别的潜在工具。
Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines (HFE145, NCI-N87, MKN28, RF1, KATO III and RF48), 16 significantly dysregulated miRNAs were found. 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the 16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis. Finally, miRNA-gene network analyses identified miRNA-125b as a crucial miRNA in GC development. Taken together, these results develop a comprehensive expression and functional profile of differentially expressed miRNAs related to gastric oncogenesis. This profile may serve as a potential tool for biomarker and therapeutic target identification in GC patients.