PROMOTIVE EFFECT ON HUMAN-SPERM PROGRESSIVE MOTILITY BY PROSTASOMES
PROMOTIVE EFFECT ON HUMAN-SPERM PROGRESSIVE MOTILITY BY PROSTASOMES
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DOI：
10.1007/bf00255932
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发表时间：
1982-01-01
影响因子：
--
通讯作者：
RONQUIST, G
中科院分区：
文献类型：
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作者：
STEGMAYR, B;RONQUIST, G
在Sephadex G200凝胶柱上分离精浆成分。分析柱洗脱液的蛋白质含量、atp酶活性及对精子进行性运动的促进作用。制备性超离心沉淀后，对两种不同的色谱组分进行了电镜观察。精子进行性运动的最大促进值与首先在色谱柱上洗脱的精浆中含有较少蛋白质的单峰中atp酶活性的最大值一致，该单峰比色谱中出现的其他峰少。第一个峰是唯一一个含有atp酶活性和被称为前列腺体的膜包围细胞器的峰。其他峰富含蛋白质，但缺乏三磷酸腺苷酶和前列腺体，在促进精子进行性运动方面表现出适度和相当不规则的模式。有证据表明，前列腺小体对精子进行性运动的积极作用是特异性的。因此，旨在改变前列腺体膜完整性的程序导致对精子进行性运动的影响减弱。这可以用钙离子的电化学梯度的可能耗散来解释。
Seminal plasma constituents were separated on Sephadex G200 gel columns. The column eluate was analysed with regard to protein content, ATPase activity and promotive activity on sperm progressive motility. Two different chromatographic fractions were also subjected to electron microscopy after sedimentation by preparative ultracentrifugation. A maximum promotive value on sperm progressive motility coincided with a maximum ATPase activity value in a single peak from seminal plasma eluted first on the column and containing less protein than the other peaks appearing later in the chromatogram. This first peak was the only one containing ATPase activity and membrane-surrounded organelles named prostasomes. Other peaks, rich in protein but lacking ATPase and prostasomes, displayed a moderate and rather irregular pattern in reference to promotive activity on sperm progressive motility. Evidence is given that the positive effect by prostasomes is specific on sperm progressive motility. Hence, procedures aiming at a change of membrane integrity of the prostasomes resulted in diminished effects on sperm progressive motility. This could be explained by a probable dissipation of the electrochemical gradient of calcium ions.