Bone marrow mononuclear cells exert long-term neuroprotection in a rat model of ischemic stroke by promoting arteriogenesis and angiogenesis.
Bone marrow mononuclear cells exert long-term neuroprotection in a rat model of ischemic stroke by promoting arteriogenesis and angiogenesis.
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DOI：
10.1016/j.bbi.2013.07.010
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发表时间：
2013-11
影响因子：
15.1
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Wang, Jianping;Yu, Lie;Jiang, Chao;Chen, Ming;Ou, Chunying;Wang, Jian
移植的骨髓来源的单核细胞（BMMNC）可以通过整合到血管壁并分化为平滑肌细胞（SMC）和内皮细胞（EC）来促进动脉生成和血管生成。在这里，我们探讨了BMMNCs是否可以增强动脉生成和血管生成，并促进长期功能恢复的大鼠模型永久性大脑中动脉闭塞（pMCAO）。在诱导pMCAO后24 h，经股静脉注射溶剂或1×107个BrdU标记的BMMNCs。在pMCAO后42天每周评估功能缺陷，并在第7天评估梗死体积。我们在第14天和第42天通过乳胶灌注可视化血管结构。与未处理或溶剂处理的缺血组相比，BMMNC移植显著减少了梗死体积和神经功能缺损。在BMMNC治疗的大鼠中，BrdU阳性细胞广泛分布在梗死边界区，被纳入血管壁，并增强软脑膜炎，Willis环和基底动脉的生长。BMMNC显示从中风后第14天起分化为SMC和EC，并保留血管修复功能至少6周。我们的数据表明，BMMNCs可以显着增强动脉生成和血管生成，减少梗死体积，并促进pMCAO后大鼠的长期功能恢复。
Transplanted bone marrow-derived mononuclear cells (BMMNCs) can promote arteriogenesis and angiogenesis by incorporating into vascular walls and differentiating into smooth muscle cells (SMCs) and endothelial cells (ECs). Here, we explored whether BMMNCs can enhance arteriogenesis and angiogenesis and promote long-term functional recovery in a rat model of permanent middle cerebral artery occlusion (pMCAO). Sprague-Dawley rats were injected with vehicle or 1×107 BMMNCs labeled with BrdU via femoral vein 24 h after induction of pMCAO. Functional deficits were assessed weekly through day 42 after pMCAO, and infarct volume was assessed on day 7. We visualized the angioarchitecture by latex perfusion on days 14 and 42. BMMNC transplantation significantly reduced infarct volume and neurologic functional deficits compared with untreated or vehicle-treated ischemic groups. In BMMNC-treated rats, BrdU-positive cells were widely distributed in the infarct boundary zone, were incorporated into vessel walls, and enhanced the growth of leptomeningeal anastomoses, the circle of Willis, and basilar arteries. BMMNCs were shown to differentiate into SMCs and ECs from day 14 after stroke and preserved vascular repair function for at least 6 weeks. Our data indicate that BMMNCs can significantly enhance arteriogenesis and angiogenesis, reduce infarct volume, and promote long-term functional recovery after pMCAO in rats.
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影响因子：
8.3
作者：
Shimada IS;Peterson BM;Spees JL
通讯作者：
Spees JL
DOI：
10.1161/01.atv.0000179768.06206.cb
发表时间：
2005-10-01
影响因子：
8.7
作者：
Imada, T;Tatsumi, T;Matsubara, H
通讯作者：
Matsubara, H
影响因子：
37.8
作者：
Simper, D;Stalboerger, PG;Caplice, NM
通讯作者：
Caplice, NM
影响因子：
20.1
作者：
Chen Z;Rubin J;Tzima E
通讯作者：
Tzima E
DOI：
10.1038/jcbfm.2009.198
发表时间：
2010-01
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
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作者：
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