Label-free quantitative proteomic analysis revealed a positive effect of ectopic over-expression of PeaT1 from Alternaria tenuissima on rice (Oryza sativa) response to drought
Label-free quantitative proteomic analysis revealed a positive effect of ectopic over-expression of PeaT1 from Alternaria tenuissima on rice (Oryza sativa) response to drought
复制标题
无标记定量蛋白质组学分析揭示了 Alternaria tenuissima 中 PeaT1 的异位过度表达对水稻 (Oryza sativa) 干旱反应的积极影响
DOI：
10.1007/s13205-018-1507-5
复制
发表时间：
2018-11-13
期刊：
影响因子：
2.8
通讯作者：
Qiu, Dewen
中科院分区：
文献类型：
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作者：
Shi, Fachao;Yang, Xiufen;Qiu, Dewen
蛋白质激发子PeaT 1存在于极细链格孢中，在小麦、棉花和水稻中表现出广谱抗性。最近，我们发现过表达PeaT 1水稻（OE）可以增强植物的耐旱性。阐明一些与干旱胁迫相关的蛋白质及其相关机制是提高水稻耐旱性的必然选择。本研究结合无标记定量蛋白质组学方法，对OE植物中多种蛋白质的差异积累进行了研究。其中，共有57个显著变化的蛋白质（包括32个上调和25个下调），主要涉及代谢、细胞、生物学进程和应激反应。使用RT-qPCR检测，检测到18个蛋白质的相对丰度与蛋白质组数据中的蛋白质丰度基本一致。特别是与非生物胁迫相关的蛋白质OsSKIPa和OsPP 2C在转基因水稻中脱水处理后早期被显著诱导，其他胁迫反应基因prohibitin蛋白、PsbP蛋白、msRB蛋白在转基因水稻中也发生了变化。这些结果表明，这些差异蛋白将有助于理解PeaT 1在水稻中的功能分子机制。
The protein elicitor PeaT1 was found in Alternaria tenuissima and exerted broad spectrum resistance in wheat, cotton, and rice. Recently, we found that overexpressing PeaT1 rice (OE) could enhance plant drought tolerance. Elucidating some elevated drought stress-related proteins and associated mechanisms is inevitable for improving drought tolerance in rice. In this study, combining a label-free quantitative proteomic method, multiple proteins were differentially accumulated in OE plants. Among these, a total of 57 significant changed proteins (including 32 up-regulated and 25 down-regulated) were mainly involved in metabolic, cellular, biological progress, and stress response. Using the RT-qPCR assay, 18 proteins’ relative abundance was detected mostly consistent with the proteins abundance in proteomic data. Specially, proteins involved in abiotic stress, such as OsSKIPa and OsPP2C, which were significantly induced in early after dehydration treatment in transgenic rice, and the other stress response genes (prohibitin protein, PsbP protein, msrB Protein) also changed in PeaT1 OE lines. Taken together, these results suggested that these differential proteins would be helpful for understanding the functional molecular mechanism of PeaT1 in rice.