Distribution and significance of interstitial fibrosis and stroma-infiltrating B cells in tongue squamous cell carcinoma.
Distribution and significance of interstitial fibrosis and stroma-infiltrating B cells in tongue squamous cell carcinoma.
复制标题
舌鳞状细胞癌间质纤维化和间质浸润B细胞的分布及意义
DOI：
10.3892/ol.2016.4184
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发表时间：
2016-03
期刊：
影响因子：
2.9
通讯作者：
Liao GQ
中科院分区：
文献类型：
--
作者：
Lao XM;Liang YJ;Su YX;Zhang SE;Zhou XI;Liao GQ
炎症和结缔组织增生经常在肿瘤微环境中被鉴定，并且已被证明是恶性生物事件的有效调节剂。然而，炎症微环境和间质纤维化相互作用的机制仍有待阐明。本研究旨在探讨舌鳞状细胞癌（TSCC）中炎症和间质纤维化的程度，以及其如何影响TSCC的预后。组织样本来自93例TSCC和配对的肿瘤相邻的非肿瘤性舌上皮，以及14例上皮异型增生，被使用。使用Masson三色染色评估间质胶原纤维。通过检测α-平滑肌肌动蛋白（SMA）、波形蛋白、结蛋白和分化簇19（CD 19），对癌相关成纤维细胞（CAF）和间质浸润B细胞进行免疫组化鉴定。统计分析TSCC患者的临床病理学意义和总生存期。在TSCC间质中发现了规则分布的CAF和CD 19 + B细胞，而在上皮异型增生样本或配对的肿瘤相邻非肿瘤性舌上皮样本中未观察到CAF或CD 19 + B细胞。间质胶原纤维和CAF的分布与原发癌的肿瘤分期密切相关，高水平的CD 19 + B细胞和低CAF浸润与TSCC的良好预后相关。总之，炎症和间质纤维化微环境在TSCC中共存，并且各自对疾病结果具有特定的影响，单独或可能共同。然而，在TSCC中微环境如何相互影响仍有待确定。
Inflammation and desmoplasia are frequently identified in the tumor microenvironment, and have been demonstrated to be effective modulators of malignant biological events. However, the mechanisms by which the inflammatory microenvironment and interstitial fibrosis interact with one another remain to be elucidated. The present study aimed to investigate the degree of inflammation and interstitial fibrosis in tongue squamous cell carcinoma (TSCC), and how this acts to affect the outcome of TSCC. Tissue samples from 93 cases of TSCC and paired tumor-adjacent non-neoplastic tongue epithelium, as well as 14 cases of epithelial dysplasia, were used. Interstitial collagen fibers were assessed using Masson's trichrome stain. Immunohistochemical identification of cancer-associated fibroblasts (CAFs) and stroma-infiltrating B cells was performed via detection of α-smooth muscle actin (SMA), vimentin, desmin and cluster of differentiation 19 (CD19). The clinicopathological significance and overall survival of the TSCC patients were statistically analyzed. Regularly distributed CAFs and CD19+ B cells were identified in the TSCC stroma, whereas no CAFs or CD19+ B cells were observed in epithelial dysplasia samples or paired tumor-adjacent non-neoplastic tongue epithelium samples. The distribution of interstitial collagen fibers and CAFs was closely associated with the tumor stage of the primary cancer, and high levels of CD19+ B cells together with low CAF infiltration were identified to be associated with favorable prognosis in TSCC. In conclusion, the inflammatory and interstitial fibrotic microenvironments coexist in TSCC, and each has specific effects on disease outcome, individually or perhaps collectively. However, it remains to be determined exactly how the microenvironments affect one another in TSCC.