Separation of zeta (zeta) and various gamma (gamma) chains of human embryonic hemoglobin Portland I by reverse-phase high-performance liquid chromatography.
Separation of zeta (zeta) and various gamma (gamma) chains of human embryonic hemoglobin Portland I by reverse-phase high-performance liquid chromatography.
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采用反相高效液相色谱法分离人胚胎血红蛋白 Portland I 的 zeta（zeta）和各种 γ（gamma）链。
DOI：
10.1016/0003-2697(83)90067-2
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发表时间：
1983
影响因子：
2.9
通讯作者：
Lie-Injo,LE
中科院分区：
文献类型：
--
作者：
Randhawa,ZI;Jones,RT;Lie-Injo,LE
从纯合型α地中海贫血引起胎儿水肿的新生儿血样中分离出Hb波特兰I的zeta（ζ）和各种γ（γ）链。使用含有乙腈、甲醇和磷酸钾的展开剂和分析型（3.9 mm × 30 cm）或制备型（7.8 mm × 30 cm）μBondapak C-18柱（沃茨），分离出200 μg至5.0 mg的纯珠蛋白链。已经开发了使用短（50分钟持续时间）和延长（186分钟持续时间）梯度程序的分析和制备程序。除色谱柱类型和显色条件外，发现以下因素也很重要：（a）样品制备，（B）样品加载，和（c）色谱柱清洁。初步研究表明，根据珠蛋白样品的类型和柱龄，产率范围为40%至60%。该方法还允许从胎儿和成人样品中分离α、β和各种γ珠蛋白链。
The zeta (ζ) and various gamma (γ) chains of Hb Portland I have been separated and isolated from blood samples obtained from neonates with hydrops fetalis due to homozygous α-thalassemia. By using developers containing acetonitrile, methanol, and potassium phosphate and either an analytical (3.9 mm × 30 cm) or a preparative (7.8 mm × 30 cm) μBondapak C-18 column (Waters), globin chains from 200 μg to 5.0 mg have been isolated in pure forms. Analytical and preparative procedures using short (50-min duration) and extended (186-min duration) gradient programs have been developed. In addition to the type of column and developer conditions, the following factors are found to be important: (a) preparation of sample, (b) sample loading, and (c) cleaning of the column. Preliminary studies indicate that the yield ranges from 40 to 60% depending on the type of globin sample and the age of the column. This procedure also permits the separation of α, β, and various γ globin chains from fetal and adult samples.