Assignment of first random restriction fragment length polymorphism (RFLP) locus ((D14S1) to a region of human chromosome 14.
Assignment of first random restriction fragment length polymorphism (RFLP) locus ((D14S1) to a region of human chromosome 14.
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DOI：
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发表时间：
1982-03
影响因子：
9.8
通讯作者：
B. de Martinville;A. Wyman;R. White;U. Francke
中科院分区：
文献类型：
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作者：
B. de Martinville;A. Wyman;R. White;U. Francke
通过将Eco - RI片段与重组质粒pAW101中的随机人DNA序列杂交，鉴定出了一个限制性片段长度多态性（RFLP）位点。我们检测了从20个人类X中国仓鼠体细胞杂交体中提取的DNA，以发现在pAW101中存在与人类插入物同源的序列。这些杂交种来自6个不同的人类捐赠者，其中5个是杂合的，在南方转移中产生了两个条带。杂种中同源序列的存在与人类14号染色体的存在完全相关。3个杂交种含有14号染色体的频率大于每个细胞1个，2个等位基因呈阳性。两个杂种只包含14个长臂的远端一半作为易位的一部分，仍然是阳性的。这些结果将第一个高度多态性随机RFPL位点（D14S1）定位在14号染色体q21区，导致四分之一。
A locus responsible for a restriction fragment length polymorphism (RFLP) has been identified by hybridization of Eco RI fragments to the random human DNA sequence in recombinant plasmid pAW101. We have examined DNA extracted from 20 human X Chinese hamster somatic cell hybrids for the presence of sequences homologous to the human insert in pAW101. The hybrids were derived from six different human donors, five of whom were heterozygous, producing two bands on Southern transfers. The presence of homologous sequences in the hybrids correlated exclusively with the presence of human chromosome 14. Three hybrids contained chromosome 14 in a frequency of greater than one per cell and were positive for two alleles. Two hybrids contained only the distal half of the long arm of 14 as part of a translocation and were still positive. These results assign the first highly polymorphic random RFPL locus (D14S1) to region q21 leads to qter of chromosome 14.