Analysis of Toxoplasma gondii antigens recognized by human sera obtained before and after acute infection.
Analysis of Toxoplasma gondii antigens recognized by human sera obtained before and after acute infection.
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急性感染前后获得的人血清识别的弓形虫抗原的分析。
DOI：
10.1093/infdis/154.4.650
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Remington,JS
中科院分区：
文献类型：
--
作者：
Potasman,I;Araujo,FG;Desmonts,G;Remington,JS
在弓形虫急性感染之前和期间获得的人血清样本中研究了对弓形虫的抗体反应。弓形虫。通过 Sabin-Feldman 染料试验、双夹心 IgM ELISA、凝集试验和弓形虫抗原蛋白印迹进行分析。研究人员对 17 名患者进行了研究：12 名孕妇、2 名心脏移植接受者和 3 名有症状但免疫正常的个体。每个患者的第一个血清学阳性血清样本的 IgM 和 IgG 蛋白印迹都显示出新的条带，以及血清学阴性血清样本中注意到的条带的强化染色。在每位患者中，对应于~4 kDa（kDa；IgM 印迹）和~35 kDa (IgG) 抗原的条带染色最深。在一名患者中，先通过蛋白质印迹证实针对 4-kDa 抗原的 IgM 抗体，然后再通过 IgM ELISA 证实。慢性感染 T 菌的血清 IgG 蛋白印迹。 gondire 显示出针对 4-kDa 和 35-kDa 抗原的弱反应性抗体；这些条带在 IgM 印迹中无法显示。
The antibody response toToxoplasma gondiiwas studied in human serum samples obtained before and during acute infection withT. gondii. Analysis was by the Sabin-Feldman dye test, double-sandwich IgM ELISA, agglutination test, and protein blots of toxoplasma antigens. Seventeen patients were studied: 12pregnant women, 2 heart transplant recipients, and 3 symptomatic but immunologically normal individuals. Both IgM and IgG protein blots of the first serologically positive serum sample from each patient revealed new bands, as well as intensified staining of bands noted with the serologically negative serum samples. In each patient, bands corresponding to antigens of ∼4 kilodaltons (kDa; IgM blots) and of ∼35 kDa (IgG) were the most intensely stained. In one patient, IgM antibodies to the 4-kDa antigen(s) were demonstrable by protein blots before they were demonstrable by IgM ELISA. IgG protein blots of sera from individuals with chronic infection withT. gondiirevealed weakly reactive antibodies to both the 4-kDa and 35-kDa antigens; these bands were not demonstrable in the IgM blots.