Attenuating effects of Granulocyte-colony stimulating factor (G-CSF) in radiation induced intestinal injury in mice
Attenuating effects of Granulocyte-colony stimulating factor (G-CSF) in radiation induced intestinal injury in mice
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DOI：
10.1016/j.fct.2012.05.059
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发表时间：
2012-09-01
影响因子：
4.3
通讯作者：
Yang, Kwangmo
中科院分区：
文献类型：
--
作者：
Kim, Joong-Sun;Ryoo, Seung-Bum;Yang, Kwangmo
胃肠道损伤是暴露于高水平辐射后死亡的主要原因，目前尚无有效的治疗方法。在这项研究中，我们检查了粒细胞集落刺激因子 (G-CSF) 减轻腹部受致死剂量 (12 Gy) 辐射的 C3H/HeN 小鼠肠道损伤并提高其存活率的能力。照射后 3 天每天皮下注射 G-CSF（100 μg/kg 体重），与注射盐水的对照相比，3.5 天时显示可改善存活率和肠道形态。 G-CSF改善的形态特征包括隐窝数量和深度、绒毛长度以及10个肠细胞的基底层长度。 G-CSF 还可使循环肿瘤坏死因子 α 的水平正常化，并减少由辐射诱导的骨髓抑制引起的外周中性粒细胞的损失。总之，我们的结果表明，G-CSF 提高了受辐射小鼠的存活率，并最大限度地减少了辐射对胃肠道损伤的影响。 (C) 2012 Elsevier Ltd. 保留所有权利。
Gastrointestinal injury is a major cause of death following exposure to high levels of radiation, and no effective treatments are currently available. In this study, we examined the capacity of granulocyte colony-stimulating factor (G-CSF) to mitigate intestinal injury in, and improve survival of, C3H/HeN mice given a lethal dose (12 Gy) of radiation to the abdomen. G-CSF (100 mu g/kg body weight) was injected subcutaneously daily for 3 days after irradiation and shown to improve survival and intestinal morphology at 3.5 days compared with saline-injected controls. The morphological features improved by G-CSF included crypt number and depth, villous length, and the length of basal lamina of 10 enterocytes. G-CSF also normalized the levels of circulating tumor necrosis factor alpha and attenuated the loss of peripheral neutrophils, caused by radiation-induced myelosuppression. In conclusion, our results suggest that G-CSF enhanced the survival of irradiated mice and minimized the effects of radiation on gastrointestinal injury. (C) 2012 Elsevier Ltd. All rights reserved.