Expression of surfactant proteins in embryonic rat lung.
Expression of surfactant proteins in embryonic rat lung.
复制标题
表面活性蛋白在大鼠胚胎肺中的表达。
DOI：
10.1165/ajrcmb.10.2.7509164
复制
发表时间：
1994
影响因子：
6.4
通讯作者：
Post,M
中科院分区：
文献类型：
--
作者：
Wang,J;Souza,P;Kuliszewski,M;Tanswell,AK;Post,M
由于表面活性剂蛋白A、B和C （SP-A、SP-B和SP-C）被认为是肺泡上皮II型细胞的标记物，我们研究了它们在胚胎大鼠肺（12至15天，足月= 22天）中的表达。通过逆转录聚合酶链反应（RT-PCR）评估SP-A、SP-B和SP-C的表达。妊娠12天的胚胎大鼠肺缺乏这三种表面活性剂蛋白的可检测mrna。然而，SP-A、SP-B和SP-C在妊娠13天的胚胎大鼠肺中存在。SP-A mRNA在胚胎大鼠肺中的表达随着妊娠的推进而增加。表面活性剂蛋白mRNA在肺发育胚胎期的表达仅限于上皮细胞。SP-A mRNA的表达虽然仅限于肺，但似乎并不是注定成为II型细胞的细胞的标志，因为它在妊娠13天的胚胎大鼠肺的气管和推定的主支气管管以及肺远端肺泡前区被检测到。SP-B和SP-C mrna在胚胎肺中的表达仅限于导管系统的远端部分，尽管SP-C的信息也在脑和肾脏中被发现。这些结果表明，在肺发育的早期阶段，所研究的三种表面活性剂蛋白mrna中没有一种对注定成为II型肺细胞的细胞具有特异性。
Because surfactant proteins A, B, and C (SP-A, SP-B, and SP-C) are putative markers for alveolar epithelial type II cells, we investigated their expression in embryonic rat lung (12 to 15 days, term= 22 days). The expression of the messages for SP-A, SP-B, and SP-C was assessed by the reverse-transcriptase polymerase chain reaction (RT-PCR). Embryonic rat lung at 12 days' gestation lacked detectable mRNAs for all three surfactant proteins. Messages for SP-A, SP-B, and SP-C were, however, present in embryonic rat lung at 13 days' gestation. Expression of SP-A mRNA increased in embryonic rat lung with advancing gestation. Surfactant protein mRNA expression during the embryonic period of lung development was limited to the epithelial cells. The expression of SP-A mRNA, while limited to the lung, did not appear to be a marker for cells destined to become type II cells, since it was detected in the trachea and the presumptive main bronchial ducts of embryonic rat lung at 13 days' gestation, as well as in the distal lung prealveolar region. Expression of both SP-B and SP-C mRNAs in embryonic lung was confined to the distal portion of the ductal system, although message of SP-C was also found in brain and kidney. These results suggest that none of the three surfactant protein mRNAs studied are, at this early stage of lung development, specific for cells destined to become type II pneumocytes.