Neurotrophic agents in fibrin glue mediate adult dorsal root regeneration into spinal cord
Neurotrophic agents in fibrin glue mediate adult dorsal root regeneration into spinal cord
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DOI：
10.1097/00006123-199903000-00085
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发表时间：
1999-03-01
期刊：
影响因子：
4.8
通讯作者：
Itoh, Y
中科院分区：
文献类型：
--
作者：
Iwaya, K;Mizoi, K;Itoh, Y
目的：本研究的目的是确定是否神经营养因子（NTFs）外源性管理的纤维蛋白胶协助切断成年大鼠背根轴突再生成spinal cord.METHODS：大鼠接受椎管内植入物的纤维蛋白胶含有神经营养因子-3，脑源性NTF，睫状神经营养因子，或Dulbecco的修改鹰的介质（对照）到左背象限腔抽吸在腰椎扩大。将横断的L5背根残端放置在病变腔的底部，并固定在纤维蛋白胶和脊髓之间。再生的背根轴突随后用免疫组织化学方法标记，以证明那些含有降钙素基因相关peptide.RESULTS：降钙素基因相关肽免疫反应性背根轴突再生的大鼠背根脊髓界面与纤维蛋白胶含有神经营养因子-3，脑源性NTF，或睫状神经营养因子，进入脊髓，并经常arborized内运动神经元细胞体的集群。只有少数轴突再生到缺乏NTF的纤维蛋白胶植入物的动物的脊髓中，并且它们在脊髓内的生长极其有限。定量研究的结果证实了这些observation.CONCLUSION：我们的研究结果表明，神经营养素-3，脑源性NTF，睫状神经营养因子增强背根再生到脊髓和纤维蛋白胶是一种有效的介质，用于椎管内传递的NTF。因此，这种递送NTF的方法可以提供用于恢复受损的脊髓反射弧的策略。
OBJECTIVE: The aim of the present study was to determine whether neurotrophic factors (NTFs) exogenously administered in fibrin glue assisted cut dorsal root axons of adult rats to regenerate into the spinal cord.METHODS: Rats received intraspinal implants of fibrin glue containing neurotrophin-3, brain-derived NTF, ciliary NTF, or Dulbecco's modified Eagle's medium (control) into left dorsal quadrant cavities aspirated in the lumbar enlargement. The transected L5 dorsal root stump was placed at the bottom of the lesion cavity and was secured between the fibrin glue and the spinal cord. Regenerated dorsal root axons were subsequently labeled with immunohistochemical methods to demonstrate those that contained calcitonin gene-related peptide.RESULTS: Calcitonin gene-related peptide-immunoreactive dorsal root axons regenerated across the dorsal root-spinal cord interface of rats with fibrin glue containing neurotrophin-3, brain-derived NTF, or ciliary NTF, entered the spinal cord, and frequently arborized within clusters of motoneuronal cell bodies. Only a few axons regenerated into the spinal cord of animals with fibrin glue implants that lacked NTF, and their growth within the spinal cord was extremely limited. The results of quantitative studies confirmed these observations.CONCLUSION: Our results indicate that neurotrophin-3, brain-derived NTF, and ciliary NTF enhance dorsal root regeneration into spinal cord and that fibrin glue is an effective medium for intraspinal delivery of NTF. This method of delivering NTF may therefore provide a strategy for restoring injured spinal reflex arcs.