Astrocytes express functional TRPV2 ion channels.
Astrocytes express functional TRPV2 ion channels.
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DOI：
10.1016/j.bbrc.2013.10.046
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发表时间：
2013-11
影响因子：
3.1
通讯作者：
K. Shibasaki;Y. Ishizaki;S. Mandadi
中科院分区：
文献类型：
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作者：
K. Shibasaki;Y. Ishizaki;S. Mandadi
温度敏感瞬时受体电位（thermoTRP）通道在感觉传导中起重要作用。其中，TRPV 2具有被非常高的温度（>52 °C）激活的有趣特性。除了热传感器功能外，TRPV 2还充当机械传感器，osomosensor和脂质传感器。据报道，TRPV2在心脏、肠、胰腺和感觉神经中表达。在中枢神经系统中，神经元TRPV2的表达被报道，然而，神经胶质细胞的表达和TRPV2的确切作用尚未确定。为探讨TRPV2在星形胶质细胞中的功能性表达，采用组织学和生理学方法检测TRPV2在星形胶质细胞中的表达。有趣的是，在星形胶质细胞的质膜中检测到TRPV2表达，并且星形胶质细胞TRPV2被非常高的温度（>50 °C）激活，与报道的特征一致。我们发现，星形胶质细胞TRPV2也被激活的溶血磷脂酰胆碱，TRPV2的一种已知的内源性脂质配体，表明星形胶质细胞TRPV2可能调节神经元的活动，响应脂质代谢。因此，我们首次揭示了TRPV2在星形胶质细胞中以及神经元中功能性表达。
Thermosensitive transient receptor potential (thermo TRP) channels are important for sensory transduction. Among them, TRPV2 has an interesting characteristic of being activated by very high temperature (>52 °C). In addition to the heat sensor function, TRPV2 also acts as a mechanosensor, an osomosensor and a lipid sensor. It has been reported that TRPV2 is expressed in heart, intestine, pancreas and sensory nerves. In the central nervous system, neuronal TRPV2 expression was reported, however, glial expression and the precise roles of TRPV2 have not been determined. To explore the functional expression of TRPV2 in astrocytes, the expression was determined by histological and physiological methods. Interestingly, TRPV2 expression was detected in plasma membrane of astrocytes, and the astrocytic TRPV2 was activated by very high temperature (>50 °C) consistent with the reported characteristic. We revealed that the astrocytic TRPV2 was also activated by lysophosphatidylcholine, a known endogenous lipid ligand for TRPV2, suggesting that astrocytic TRPV2 might regulate neuronal activities in response to lipid metabolism. Thus, for the first time we revealed that TRPV2 is functionally expressed in astrocytes in addition to neurons.