Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways
Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways
复制标题
有机硒通过抑制NF-κB和MAPK信号通路的激活改善金黄色葡萄球菌诱导的大鼠乳腺炎
DOI：
10.3389/fvets.2020.00443
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发表时间：
2020-07-30
影响因子：
3.2
通讯作者：
Li, Jianji
中科院分区：
文献类型：
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作者：
Liu, Kangjun;Ding, Tao;Li, Jianji
奶牛乳房炎是奶牛的一种重要的经济疾病，常由金黄色葡萄球菌（S。 aureus)具有良好的抗菌活性。硒是生理功能不可缺少的元素，有助于减少乳腺炎时乳腺的损伤。然而，充足的硒源一直是家畜的重要考虑因素。因此，本研究旨在探讨硒化高羊毛硫氨酸（SeHLan）对S.金黄色。色葡萄建立金黄色葡萄球菌诱导的大鼠模型，并在饲料中添加三个剂量（0.2、2、20 μ g/kg体重/天）的OS。对乳腺进行细菌载量、组织病理学检查和髓过氧化物酶（MPO）测定。使用qRT-PCR检测细胞因子，包括白细胞介素（IL）-1 β、TNF-α和IL-6。Western blot分析NF-κ B和MAPK信号通路的关键蛋白。结果表明，添加OS可减少中性粒细胞和巨噬细胞在乳腺组织中的募集，但不降低S.金黄色葡萄球菌在组织中的负载。IL-1 β、TNF-α和IL-6在S. OS处理后对金黄色葡萄球菌均有抑制作用。此外，NF-κ B和MAPKs蛋白的磷酸化增加也受到抑制。结果提示，孕期补充适量的OS有助于保护乳腺免受S.并减轻炎症反应。
Mastitis is an economically important disease in dairy cows, which is often caused by Staphylococcus aureus (S. aureus). Selenium is an indispensable element for physiological function and contributes to reduce injury of the mammary glands in mastitis. However, adequate sources of selenium have always been an important consideration for livestock. Therefore, the study aimed to explore the protective effect and mechanism of Selenohomolanthionine (SeHLan) on mastitis induced by S. aureus. The S. aureus-induced rat model was established and three doses (0.2, 2, 20 mu g/kg body weight/day) of dietary OS were supplemented. The bacterial load, histopathology, and myeloperoxidase (MPO) of the mammary glands were performed and determined. Cytokines, including interleukin (IL)-1 beta, TNF-alpha, and IL-6, were detected using qRT-PCR. The key proteins of NF-kappa B and MAPK signaling pathways were analyzed by Western blot. The results revealed that OS supplementation could reduce the recruitment of neutrophils and macrophages in mammary tissues, but did not decrease S. aureus load in the tissues. The overexpression levels of IL-1 beta, TNF-alpha, and IL-6 induced by S. aureus were inhibited after OS treatment. Furthermore, the increased phosphorylation of NF-kappa B and MAPKs proteins were also suppressed. The results suggest that dietary supplementation with adequate OS during pregnancy contributes to protect the mammary glands from injury caused byS. aureus and alleviate the inflammatory response.