The national DBS brain tissue network pilot study: need for more tissue and more standardization.
The national DBS brain tissue network pilot study: need for more tissue and more standardization.
复制标题
国家DBS脑组织网络试点研究：需要更多组织和更多标准化。
DOI：
10.1007/s10561-010-9189-1
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发表时间：
2011
影响因子：
1.5
通讯作者：
Okun,MS
中科院分区：
文献类型：
--
作者：
Vedam-Mai,V;Krock,N;Ullman,M;Foote,KD;Shain,W;Smith,K;Yachnis,AT;Steindler,D;Reynolds,B;Merritt,S;Pagan,F;Marjama-Lyons,J;Hogarth,P;Resnick,AS;Zeilman,P;Okun,MS
全球已植入超过70,000台DBS设备；然而，可供研究人员使用的、经过充分表征的死后 DBS 大脑仍然很少。我们建议通过建立深部脑刺激-脑组织网络（DBS-BTN）可以提高对DBS的整体理解，这将进一步加深我们对DBS和大脑功能的理解。组织库的目标有两个：(a) 为 DBS 脑组织样本提供完整的（临床、成像和病理）数据库；(b) 向研究人员提供 DBS 组织样本，这将有助于我们了解疾病和潜在的大脑回路。作为试点项目的一部分，开发了处理 DBS 大脑的标准操作程序。为个体患者创建完整的数据文件，包括人口统计信息、临床信息、影像数据、病理学和 DBS 引导位置/设置。 19 个 DBS 大脑是从美国各地 11 个地理分散的中心收集的。死亡时的平均年龄为 69.3 岁（51-92 岁，标准差或 SD 为 10.13）。男女比例接近3:1。从死亡到大脑采集的平均尸检间隔为 10.6 小时（标准差为 7.17）。 DBS 目标包括：丘脑底核、苍白球内核和丘脑腹中间核。 16.7%的病例临床诊断与病理诊断不符。我们提供该队列的神经病理学发现以及 DBS 的围导期反应。该试点研究中最重要的观察结果之一是缺失数据，该数据约占所有可用数据字段的 25%。初步结果证明了为科学界创建国家 DBS-BTN 资源的可行性和实用性。我们计划改进我们的技术来弥补遗漏的临床/研究数据，并扩大网络以包括更大的捐赠者库。我们将加强样品制备，以促进先进的分子研究和祖细胞修复。
Over 70,000 DBS devices have been implanted worldwide; however, there remains a paucity of well-characterized post-mortem DBS brains available to researchers. We propose that the overall understanding of DBS can be improved through the establishment of a Deep Brain Stimulation-Brain Tissue Network (DBS-BTN), which will further our understanding of DBS and brain function. The objectives of the tissue bank are twofold: (a) to provide a complete (clinical, imaging and pathological) database for DBS brain tissue samples, and (b) to make available DBS tissue samples to researchers, which will help our understanding of disease and underlying brain circuitry. Standard operating procedures for processing DBS brains were developed as part of the pilot project. Complete data files were created for individual patients and included demographic information, clinical information, imaging data, pathology, and DBS lead locations/settings. 19 DBS brains were collected from 11 geographically dispersed centers from across the U.S. The average age at the time of death was 69.3 years (51–92, with a standard deviation or SD of 10.13). The male:female ratio was almost 3:1. Average post-mortem interval from death to brain collection was 10.6 h (SD of 7.17). The DBS targets included: subthalamic nucleus, globus pallidus interna, and ventralis intermedius nucleus of the thalamus. In 16.7% of cases the clinical diagnosis failed to match the pathological diagnosis. We provide neuropathological findings from the cohort, and perilead responses to DBS. One of the most important observations made in this pilot study was the missing data, which was approximately 25% of all available data fields. Preliminary results demonstrated the feasibility and utility of creating a National DBS-BTN resource for the scientific community. We plan to improve our techniques to remedy omitted clinical/research data, and expand the Network to include a larger donor pool. We will enhance sample preparation to facilitate advanced molecular studies and progenitor cell retrieval.