5-AZACYTIDINE PERMITS GENE ACTIVATION IN A PREVIOUSLY NONINDUCIBLE CELL TYPE
5-AZACYTIDINE PERMITS GENE ACTIVATION IN A PREVIOUSLY NONINDUCIBLE CELL TYPE
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DOI：
10.1016/0092-8674(85)90155-2
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
BLAU, HM
中科院分区：
文献类型：
--
作者：
CHIU, CP;BLAU, HM
成纤维细胞中的沉默肌肉基因在与肌肉细胞融合形成异核体后可被激活。这种激活不需要染色质结构的改变，包括显著的DNA合成。当HeLa细胞用作非肌肉融合伙伴时，从未观察到肌肉基因激活。如果在融合前用5-氮杂胞苷（5-aza-CR）处理HeLa细胞，则在异核体中诱导肌肉基因表达。早期（5.1H11细胞表面抗原）和晚期（mm -肌酸激酶）肌肉功能的基因都被激活，但经常不协调表达。两个肌肉基因的表达通常是顺序的，但显然不是相互依赖的。5-aza-CR诱导的变化（可能是DNA甲基化水平的变化）是HeLa细胞中肌肉基因响应肌肉细胞中存在的假定的反式作用调节因子而表达所必需的。
Silent muscle genes in fibroblasts could be activated following fusion with muscle cells to form heterokaryons. This activation did not require changes in chromatin structure involving significant DNA synthesis. Muscle gene activation was never observed when HeLa cells were used as the nonmuscle fusion partner. If HeLa cells were treated with 5-azacytidine (5-aza-CR) prior to fusion, muscle gene expression was induced in the heterokaryons. The genes for both an early (5.1H11 cell surface antigen) and a late (MM-creatine kinase) muscle function were activated, but were frequently not coordinately expressed. The exprssion of 2 muscle genes, which is usually sequential, evidently is not interdependent. Changes induced by 5-aza-CR, presumably in the level of DNA methylation, are required for muscle genes in HeLa cells to be expressed in response to putative trans-acting regulatory factor(s) present in muscle cells.