A Novel Marker for Undifferentiated Human Embryonic Stem Cells
A Novel Marker for Undifferentiated Human Embryonic Stem Cells
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DOI：
10.1089/mab.2014.0075
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发表时间：
2015-02-01
影响因子：
--
通讯作者：
Saito, Koichi
中科院分区：
文献类型：
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作者：
Higashi, Kiyoshi;Yagi, Masaki;Saito, Koichi
人胚胎干细胞（human embryonic stem cells，hESC）是来源于早期胚胎的多能干细胞，其自我更新能力依赖于hESC特异性分子的持续表达和分化相关基因的抑制表达。为了发现在hESC上表达的新分子，我们产生了一组针对未分化hESC的单克隆抗体。由MAb 2识别的抗原在未分化的hESC的细胞表面上表达;在hESC分化成神经细胞期间，hESC的裂解物中分子量在30和60 kDa之间的三个扩散带减少。在肺癌细胞的质膜上也观察到MAb 2抗原的表达，并且MAb 2在细胞裂解物中检测到55、50和35 kDa的蛋白条带。免疫沉淀，然后蛋白质组学分析确定CD 147/basigin作为MAb 2抗原。最后，证实了未分化hESCs中CD 147/basigin蛋白的阳性表达。这些结果表明，CD 147/basigin可能是另一个未分化hESC标记。
Human embryonic stem cells (hESCs) are pluripotent stem cells from early embryos, and their self-renewal capacity depends on the sustained expression of hESC-specific molecules and the suppressed expression of differentiation-associated genes. To discover novel molecules expressed on hESCs, we generated a panel of monoclonal antibodies against undifferentiated hESCs. The antigen recognized by MAb2 is expressed on the cell surface of undifferentiated hESCs; three diffused bands with molecular mass between 30 and 60 kDa in the lysates of hESCs were diminished during hESC differentiation into neural cells. The expression of MAb2 antigen was also observed on the plasma membrane of lung cancer cells, and MAb2 detected 55, 50, and 35 kDa protein bands in the cell lysates. Immunoprecipitation followed by proteomics analyses identified CD147/basigin as a MAb2 antigen. Finally, the positive expression of CD147/basigin protein in undifferentiated hESCs was confirmed. These results suggested that CD147/basigin could be another undifferentiated hESC marker.