Recent advances in the MS analysis of glycoproteins: Theoretical considerations.
Recent advances in the MS analysis of glycoproteins: Theoretical considerations.
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DOI：
10.1002/elps.201000393
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发表时间：
2011-01
期刊：
影响因子：
2.9
通讯作者：
Kabulski, Jarod L.
中科院分区：
文献类型：
--
作者：
Lazar, Iulia M.;Lazar, Alexandru C.;Cortes, Diego F.;Kabulski, Jarod L.
蛋白质糖基化参与调节蛋白质功能和控制细胞命运的广泛生物过程。由于已发现异常糖基化与许多疾病有关，因此蛋白质糖基化的研究和大规模表征不仅对生物学和生物医学研究界，而且对制药和生物技术工业都具有极大的兴趣。由于蛋白质/肽和聚糖部分的复杂化学结构和不同的化学性质，糖蛋白的分析和结构表征已被证明是一项困难的任务。糖基化过程本身的动态结果，以及偶尔生物样品中糖蛋白丰度低，进一步限制了大规模的努力。质谱（MS）仪器的最新进展，以及用于样品处理、富集和分离的小型化技术的进展，已经产生了强大而引人注目的分析策略，有效地解决了糖蛋白组的挑战。这篇综述总结了参与开发有效的糖蛋白组学分析方法的关键步骤，以及导致糖蛋白及其相应聚糖表征成功策略的最新创新。作为这项工作的后续行动，我们回顾了创新的毛细管和微流控MS工作流程，用于糖缀合物的鉴定，测序和表征。
Protein glycosylation is involved in a broad range of biological processes that regulate protein function and control cell fate. As aberrant glycosylation has been found to be implicated in numerous diseases, the study and large-scale characterization of protein glycosylation is of great interest not only to the biological and biomedical research community, but also to the pharmaceutical and biotechnology industry. Due to the complex chemical structure and differing chemical properties of the protein/peptide and glycan moieties, the analysis and structural characterization of glycoproteins has been proven to be a difficult task. Large-scale endeavors have been further limited by the dynamic outcome of the glycosylation process itself, and, occasionally, by the low abundance of glycoproteins in biological samples. Recent advances in mass spectrometry (MS) instrumentation, and progress in miniaturized technologies for sample handling, enrichment and separation, have resulted in robust and compelling analysis strategies that effectively address the challenges of the glycoproteome. This review summarizes the key steps that are involved in the development of efficient glycoproteomic analysis methods, and the latest innovations that led to successful strategies for the characterization of glycoproteins and their corresponding glycans. As a follow-up to this work, we review innovative capillary and microfluidic-MS workflows for the identification, sequencing, and characterization of glycoconjugates.
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影响因子：
3.4
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
4.4
作者：
Cao, Jing;Shen, Chengping;Yang, Pengyuan
通讯作者：
Yang, Pengyuan
影响因子：
2
作者：
Alley, William R., Jr.;Mechref, Yehia;Novotny, Milos V.
通讯作者：
Novotny, Milos V.