Tissue-specific expression of rat mRNAs homologous to cytochromes P-450b and P-450e.
Tissue-specific expression of rat mRNAs homologous to cytochromes P-450b and P-450e.
复制标题
与细胞色素 P-450b 和 P-450e 同源的大鼠 mRNA 的组织特异性表达。
DOI：
10.1093/nar/14.3.1525
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发表时间：
1986
影响因子：
14.9
通讯作者：
Omiecinski,CJ
中科院分区：
文献类型：
--
作者：
Omiecinski,CJ
用合成的18-mer寡聚体探针在对照和诱导剂预处理的成年大鼠的肝、肺、肾和睾丸中检查细胞色素P-450 b和P-450 e mRNA的组织特异性表达。与P-450 e探针同源的RNA在对照组和3-甲基胆蒽（MC）诱导的肝脏中检测到痕量，在苯巴比妥（PB）诱导的动物肝脏中检测到高水平。P-450 e mRNA水平低于检测限的其他组织检查，无论预处理。与此相反，在对照和诱导剂预处理的肺和睾丸中检测到低水平的与P-450 b寡聚体同源的mRNA，而在PB诱导的肝脏中检测到高水平的mRNA。在这些试验中，在来自肾脏或对照或MC预处理肝脏的RNA分离物中未检测到P-450 b mRNA。溶液杂交数据表明，大鼠肺和睾丸分别含有PB诱导的肝脏中测得的P-450 b mRNA水平的9- 12%和6- 9%。寡聚（dT）-纤维素和聚（U）-亲和实验的结果表明，P-450 b和P-450 e的肝脏mRNA主要存在于结合的聚腺苷酸化组分中，而同源肺和睾丸P-450 b mRNA主要存在于流过组分中。
The tissue-specific expression of cytochrome P-450b and P-450e mRNAs was examined with synthetic 18-mer oligomer probes in the liver, lung, kidney, and testis of control and inducer pretreated adult rats. RNAs homologous to the P-450e probe were detected in trace amounts in control and 3-methylcholanthrene (MC) induced livers and at high levels in livers from phenobarbital (PB) induced animals. P-450e mRNA levels were below detection limits in the other tissues examined, regardless of pretreatment. In contrast, mRNAs homologous to the P-450b oligomer were detected at low levels in control and inducer pretreated lung and testis, and at high levels in PB induced liver. No P-450b mRNAs were detected in these assays in RNA isolates from the kidney or from control or MC pretreated liver. Solution hybridization data indicated that the rat lung contained 9–12%, and the testis, 6–9%, respectively, of the levels of P-450b mRNA measured in the PB induced liver. Results from oligo(dT)-cellulose and poly(U)-affinity experiments indicated that the hepatic mRNAs for P-450b and P-450e were present predominantly in the bound, polyadenylated fraction, whereas the homologous lung and testes P-450b mRNAs predominated in the flow-thru fractions.