Immunohistochemical localization of 17 beta-hydroxysteroid dehydrogenase in the human endometrium during the menstrual cycle.
Immunohistochemical localization of 17 beta-hydroxysteroid dehydrogenase in the human endometrium during the menstrual cycle.
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月经周期期间人子宫内膜中 17 β-羟基类固醇脱氢酶的免疫组织化学定位。
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
R. Vihko
中科院分区：
文献类型：
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作者：
O. Mäentausta;R. Sormunen;V. Isomaa;V. Lehto;P. Jouppila;R. Vihko
免疫亲和纯化的多克隆抗兔抗人胎盘17 β-羟基类固醇脱氢酶（17 HSD）的抗体被用来定位17 HSD在冷冻切片的21人子宫内膜组织标本，采取在不同阶段的月经周期，并在人胎盘。雌激素和孕激素受体的存在和分布也进行了分析，在子宫内膜标本使用商业免疫组织化学技术。此外，17 HSD定位在子宫内膜和胎盘的免疫电镜。在子宫内膜中，17 HSD的免疫染色出现在表面上皮细胞和腺细胞的细胞质中的早期和中期的黄体期。在黄体晚期，它逐渐消失。在月经周期的卵泡期子宫内膜中未观察到免疫染色。放射免疫分析显示，17 HSD染色强度的变化与血清孕酮浓度的变化有关。17 HSD的表达与雌激素和孕激素受体的浓度呈明显的负相关。这些结果强烈支持孕酮诱导人子宫内膜腺上皮细胞和表面上皮细胞中17 HSD量增加的概念。在人类足月胎盘中，17 HSD免疫染色仅在合胞体滋养层细胞的细胞质中检测到。在子宫内膜和胎盘的免疫电镜研究中，在细胞质中观察到17 HSD染色，并且其与与内质网无关的细胞质膜相关。
Immunoaffinity-purified polyclonal anti-rabbit antibody against human placental 17 beta-hydroxysteroid dehydrogenase (17HSD) was used to localize 17HSD in frozen sections of 21 human endometrial tissue specimens, taken at different stages of the menstrual cycle, and in the human placenta. The presence and distribution of estrogen and progesterone receptors were also analyzed in endometrial specimens using commercial immunohistochemical techniques. In addition, 17HSD was localized by immunoelectron microscopy in the endometrium and the placenta. In the endometrium, immunostaining of 17HSD appeared in the cytoplasm of surface epithelial and gland cells during the early and midluteal phase. During the late luteal phase, it gradually disappeared. No immunostaining was observed in the endometrium during the follicular phase of the menstrual cycle. The changes in staining intensity of 17HSD were associated with changes in the concentration of serum progesterone as judged by radioimmunoassay. An apparent inverse correlation between 17HSD expression and the concentrations of estrogen and progesterone receptors was observed. These results strongly support the concept that progesterone induces an increase in the amount of 17HSD, in the glandular and surface epithelial cells of the human endometrium. In the human term placenta, 17HSD immunostaining was detected exclusively in the cytoplasm of syncytiotrophoblasts. In immunoelectron microscopic studies of the endometrium and placenta, 17HSD staining was observed in the cytoplasm and it was associated with cytoplasmic membranes unrelated to the endoplasmic reticulum.