Chard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increasing GLUT2 through Akt2 and antioxidant defense in the liver of rats
Chard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increasing GLUT2 through Akt2 and antioxidant defense in the liver of rats
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DOI：
10.1016/j.acthis.2013.04.016
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Yanardag, Refiye
中科院分区：
文献类型：
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作者：
Gezginci-Oktayoglu, Selda;Sacan, Ozlem;Yanardag, Refiye
甜菜是土耳其糖尿病患者用作替代降糖药的植物。本研究旨在探讨甜菜提取物降血糖作用的分子机制。将雄性Sprague-Dawley大鼠（6-7月龄）分为5组进行研究：（1）对照组，（2）高血糖组，（3）高血糖+甜菜组，（4）高血糖+胰岛素组，（5）高血糖+甜菜+胰岛素组。通过腹膜内注射60 mg/kg链脲佐菌素使动物高血糖14天后，施用甜菜水提取物（2 g/kg/天）或/和胰岛素（6 U/kg/天）45天。甜菜提取物的降血糖作用通过显著降低空腹血糖和增加甜菜提取物处理的高血糖大鼠的肝脏中的糖原水平来证明。此外，腺苷脱氨酶的活性，这被认为是一个重要的酶调节胰岛素的生物活性，被甜菜处理降低。免疫组化分析显示，甜菜或/和胰岛素治疗的高血糖大鼠的肝细胞中Akt 2的核转位和GLUT 2的合成增加。甜菜提取物或/和胰岛素治疗高血糖大鼠，通过降低丙二醛的生成，降低过氧化氢酶、超氧化物歧化酶、髓过氧化物酶的活性，提高谷胱甘肽水平，降低氧化应激，增强抗氧化防御能力。这些发现表明，甜菜提取物可能通过Akt 2和肝脏中的抗氧化防御增加GLUT 2来改善葡萄糖反应。(C)2013 Elsevier GmbH. All rights reserved.
Chard is a plant used as an alternative hypoglycemic agent by diabetic people in Turkey. The aim of this study was to examine the molecular mechanism of hypoglycemic effects of chard extract. Male Sprague-Dawley rats (6-7 months old) were divided into five groups for this investigation: (1) control, (2) hyperglycemic, (3) hyperglycemic+chard, (4) hyperglycemic + insulin, (5) hyperglycemic + chard + insulin. Fourteen days after animals were rendered hyperglycemic by intraperitoneal injection of 60 mg/kg streptozotocin, the chard water extract (2 g/kg/day) or/and insulin (6 U/kg/day) was administered for 45 days. Hypoglycemic effect of chard extract was demonstrated by a significant reduction in the fasting blood glucose and increased glycogen levels in liver of chard extract-treated hyperglycemic rats. Moreover, activity of adenosine deaminase, which is suggested as an important enzyme for modulating the bioactivity of insulin, was decreased by chard treatment. Immunostaining analysis showed increased nuclear translocation of Akt2 and synthesis of GLUT2 in the hepatocytes of chard or/and insulin-treated hyperglycemic rats. The oxidative stress was decreased and antioxidant defense was increased by chard extract or/and insulin treatment to hyperglycemic rats according to the decreased malondialdehyde formation, the activities of catalase, superoxide dismutase, myeloperoxidase and increased glutathione levels. These findings suggest that chard extract might improve glucose response by increasing GLUT2 through Akt2 and antioxidant defense in the liver. (C) 2013 Elsevier GmbH. All rights reserved.