Multiple sclerosis
Multiple sclerosis
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DOI：
10.1212/wnl.30.3.240
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发表时间：
1980-03
期刊：
影响因子：
9.9
通讯作者：
W. Tourtellotte;A. Potvin;J. Fleming;Kolar Murthy;J. Levy;K. Syndulko;J. Potvin
中科院分区：
文献类型：
--
作者：
W. Tourtellotte;A. Potvin;J. Fleming;Kolar Murthy;J. Levy;K. Syndulko;J. Potvin
基于控制白蛋白和 IgG 穿过血脑屏障 (BBB) 的生理原理，我们开发了一个经验公式来计算中枢神经系统 (CNS) 中 IgG 的从头合成速率。为了验证该公式，在 21 天内，对 9 名确诊多发性硬化症 (MS) 患者的血液和脑脊液 (CSF) 中收集了放射性标记的 IgG 和白蛋白。通过同位素交换法计算 IgG 合成率，并与经验公式获得的值进行比较。从每天 5 毫克的低合成率到每天 120 毫克的高合成率，具有极好的一致性。对两名患者进行的双重放射性标记 IgG 实验表明，MS BBB 处理正常血清 IgG 的方式与源自自体 MS 血清的 IgG 相同。因此，该经验公式仅需要一份脑脊液样本和匹配血清，就可以可靠有效地估计 MS 患者 CNS 中 IgG 的从头合成率。
An empirical formula has been developed to calculate the de novo rate of synthesis of IgG in the central nervous system (CNS), based on physiologic principles that govern the passage of albumin and IgG across the blood-brain barrier (BBB). To validate the formula, radiolabeled IgG and albumin from pooled normal t he followed from the blood to the cerebrospinal fluid (CSF) over 21 days in nine patients with definite multiple sclerosis (MS). IgG synthesis rates were calculated by the isotope exchange method and compared to values obtained with the empirical formula. There was excellent concordance, from a low rate of synthesis of 5 mg per day to a high rate of 120 mg per day. A double radiolabeled IgG experiment in two patients showed that the MS BBB processed normal serum IgG in the same way as IgG derived from autologous MS serum. Accordingly, the empirical formula, which requires only one sample of CSF and matched serum, can reliably and validly estimate the de novo rate of IgG synthesis in CNS of patients with MS.