Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh)
Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh)
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DOI：
10.1007/s10681-005-6805-4
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发表时间：
2005-01-01
期刊：
影响因子：
1.9
通讯作者：
Sansavini, S
中科院分区：
文献类型：
--
作者：
Costa, F;Stella, S;Sansavini, S
货架期决定了成熟苹果的经济寿命，可以新鲜收获或储存。良好的货架期与室温下水果硬度的缓慢下降密切相关。苹果是一种跃变型水果，其中硬度的损失似乎与乙烯有关。乙烯的生物合成途径由编码1-氨基环丙烷-1-羧酸合成酶（ACS）和1-氨基环丙烷-1-羧酸氧化酶（ACO）的两个大基因家族控制，本研究利用基因特异性分子标记检测了一个ACS和一个ACO基因对苹果乙烯产生和货架期的影响，并在分子标记连锁图谱上进行了定位。在本研究中开发了ACO标记，并将其定位在杂交组合Prima x Fiesta和Fuji x Mondial Gala的连锁群（LG）10上，在先前鉴定的果实硬度QTL的5%边界内[Theor Appl Genet 100（2000）1074]。我们将该位点标记为Md-ACO 1。此外，我们还将已开发的Md-ACS 1标记[Theor Appl Genet 101（2000）742]定位在LG 15上。对富士× Braeburn杂交的研究表明，Md-ACS 1和Md-ACO 1独立影响苹果的内部乙烯浓度（IEC）和货架期，其中Md-ACSI的影响最大。Md-ACS 1 -2和Md-ACO 1 -1纯合子后代表现出最低的乙烯产生以及优越的上级货架期。这两个基因是标记辅助育种的候选基因。
Shelf life determines the economic life time of mature apples, which can be either freshly harvested or stored. Good shelf life is highly associated with a slow decrease of fruit firmness at room temperature. Apple is a climacteric fruit, in which loss of firmness seems to be physiologically related to ethylene. Ethylene's biosynthetic pathway is controlled by two large gene families coding for 1-aminocyclopropane-1-carboxylate synthase (ACS) and 1-aminocyclopropane-1-carboxyl ate oxydase (ACO).In this study, one ACS and one ACO gene were examined for their effect on ethylene production and shelf life in apple using gene specific molecular marker, and have also been positioned on a molecular marker linkage map. The ACO marker was developed in this research and mapped on linkage group (LG) 10 of the crosses Prima x Fiesta and Fuji x Mondial Gala, within the 5% border of a previously identified fruit firmness QTL [Theor Appl Genet 100 (2000) 1074]. We denoted this locus as Md-ACO1. In addition, we mapped the previously developed Md-ACS1 marker [Theor Appl Genet 101 (2000) 742] on LG15.Studies on the cross Fuji x Braeburn revealed that Md-ACS1 and Md-ACO1 independently affect the internal ethylene concentration (IEC) as well as shelf life of apple, Md-ACSI having the strongest effect. Descendants homozygous for Md-ACS1-2 and Md-ACO1-1 showed to have the lowest ethylene production as well as superior shelf-life. These two genes are candidates to be included in marker assisted breeding.