Synergistic inhibition of human immunodeficiency virus type 1 and type 2 replication in vitro by castanospermine and 3'-azido-3'-deoxythymidine.
Synergistic inhibition of human immunodeficiency virus type 1 and type 2 replication in vitro by castanospermine and 3'-azido-3'-deoxythymidine.
复制标题
Castanospermine 和 3-azido-3-deoxythymidine 在体外协同抑制人类免疫缺陷病毒 1 型和 2 型复制。
DOI：
10.1128/aac.33.1.53
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发表时间：
1989
影响因子：
4.9
通讯作者：
Hirsch,MS
中科院分区：
文献类型：
--
作者：
Johnson,VA;Walker,BD;Barlow,MA;Paradis,TJ;Chou,TC;Hirsch,MS
研究了Castanospermine与3′-叠氮-3′-脱氧胸腺嘧啶（zidovudine）联合抗人类免疫缺陷病毒（HIV）的体外复制作用。castanosperine和3'-叠氮-3'-脱氧胸腺嘧啶在急性感染的H9细胞中协同抑制HIV 1型。此外，它们在外周血单核细胞中协同抑制HIV 1型和HIV 2型。这些药物联合使用没有额外的毒性作用。采用中效原理和等线图技术评价药物相互作用。糖基化抑制剂（如castanospermine）与3'-叠氮-3'-脱氧胸腺嘧啶的联合治疗值得考虑用于hiv相关的化疗干预。
Castanospermine and 3'-azido-3'-deoxythymidine (zidovudine) were evaluated in combination against human immunodeficiency virus (HIV) replication in vitro. Castanospermine and 3'-azido-3'-deoxythymidine inhibited HIV type 1 synergistically in acutely infected H9 cells. In addition, they synergistically inhibited both HIV type 1 and HIV type 2 in peripheral blood mononuclear cells. There were no additional toxic effects of these agents in combination. Drug interactions were evaluated by the median-effect principle and the isobologram technique. Combinations of a glycosylation inhibitor, such as castanospermine, with 3'-azido-3'-deoxythymidine deserve consideration for HIV-related chemotherapeutic intervention.