Differential Effect of DDT, DDE, and DDD on COX-2 Expression in the Human Trophoblast Derived HTR-8/SVneo Cells
Differential Effect of DDT, DDE, and DDD on COX-2 Expression in the Human Trophoblast Derived HTR-8/SVneo Cells
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DOI：
10.1002/jbt.21444
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发表时间：
2012-11-01
影响因子：
3.6
通讯作者：
Arechavaleta-Velasco, Fabian
中科院分区：
文献类型：
--
作者：
Dominguez-Lopez, Pablo;Diaz-Cueto, Laura;Arechavaleta-Velasco, Fabian
本研究的目的是研究1，1，1-三氯-2，2-二（氯苯基）乙烷（DDT）、1，1-二（氯苯基）-2，2-二氯乙烯（DDE）和1，1-二氯-2，2-二（氯苯基）乙烷（DDD）异构体对人滋养层细胞系中考克斯-2表达的影响。将培养的HTR-8/SVneo滋养层细胞暴露于DDT异构体及其代谢产物24 h，通过RT-PCR、Western blotting和ELISA评估考克斯-2 mRNA和蛋白表达。还通过ELISA测量前列腺素E2的产生。在对照（未暴露）条件下，在HTR-8/SVneo细胞系中检测到考克斯-2 mRNA和蛋白。考克斯-2蛋白表达和前列腺素E2的生产，但没有考克斯-2 mRNA水平增加后，DDE和DDD异构体暴露。结论：DDE和DDD暴露诱导考克斯-2蛋白表达，导致前列腺素E2产生增加。有趣的是，这些有机氯农药对考克斯-2的调节似乎是在翻译水平上。(c)2012 Wiley Periodicals，Inc. J Biochem Mol Toxicol 26：454-460，2012;在wileyonlinelibrary.com在线查看这篇文章。DOI 10：1002/jbt.21444
The purpose of this study was to investigate the effect of 1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane (DDT), 1,1-bis-(chlorophenyl)-2,2-dichloroethene (DDE), and 1,1-dichloro-2,2-bis(chlorophenyl)ethane (DDD) isomers on COX-2 expression in a human trophoblast-derived cell line. Cultured HTR-8/SVneo trophoblast cells were exposed to DDT isomers and its metabolites for 24 h, and COX-2 mRNA and protein expression were assessed by RT-PCR, Western blotting, and ELISA. Prostaglandin E2 production was also measured by ELISA. Both COX-2 mRNA and protein were detected under control (unexposed) conditions in the HTR-8/SVneo cell line. COX-2 protein expression and prostaglandin E2 production but not COX-2 mRNA levels increased only after DDE and DDD isomers exposure. It is concluded that DDE and DDD exposure induce the expression of COX-2 protein, leading to increased prostaglandin E2 production. Interestingly, the regulation of COX-2 by these organochlorines pesticides appears to be at the translational level. (c) 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:454-460, 2012; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.21444