Independent roles of macrophage migration inhibitory factor and endogenous, but not exogenous glucocorticoids in regulating leukocyte trafficking.
Independent roles of macrophage migration inhibitory factor and endogenous, but not exogenous glucocorticoids in regulating leukocyte trafficking.
复制标题
巨噬细胞迁移抑制因子和内源性的独立作用，但不是外源糖皮质激素在调节白细胞运输中的作用。
DOI：
10.3109/10739680903210421
复制
发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
Hickey MJ
中科院分区：
文献类型：
--
作者：
Gregory JL;Hall P;Leech M;Morand EF;Hickey MJ
巨噬细胞移动抑制因子(MIF)促进白细胞募集，拮抗糖皮质激素(GC)的抗炎作用。这项研究的目的是检验MIF和GC之间的相互作用是否支持MIF促进白细胞-内皮细胞相互作用的能力。活体显微镜被用来评估野生型和MIF−/−小鼠在内毒素、GC地塞米松治疗和使用GC受体拮抗剂RU486抑制内源性GC后的白细胞-内皮细胞相互作用。在野生型小鼠中，地塞米松将脂多糖诱导的白细胞相互作用降低到与未经地塞米松治疗的MIF−/−小鼠相似的水平，而在MIF−/−小鼠中，地塞米松不能进一步抑制白细胞相互作用。在野生型和MIF−/−小鼠中，RU486对脂多糖诱导的白细胞黏附和迁移具有相似的促进作用，提示内源性GC对野生型和MIF−/−小鼠的白细胞迁移具有相似的抑制作用。MIF缺乏和RU486处理均降低VCAM-1的表达，但均不影响ICAM-1或趋化因子CCL2、KC和MIP-2的表达。这些结果表明，内源性MIF和GC在体内通过主要独立的机制相互调节白细胞与内皮细胞的相互作用，MIF缺乏的抗炎作用与外源性GC相当。
Macrophage migration inhibitory factor (MIF) promotes leukocyte recruitment and antagonizes the anti-inflammatory effects of glucocorticoids (GC). The aim of this study was to examine whether interaction between MIF and GC underlies the ability of MIF to promote leukocyte-endothelial cell interactions. Intravital microscopy was used to assess leukocyte-endothelial cell interactions in wild-type and MIF−/− mice following treatment with LPS, the GC dexamethasone, and inhibition of endogenous GC using the GC receptor antagonist, RU486. Dexamethasone reduced LPS-induced leukocyte interactions in wild-type mice to levels similar to those observed in MIF−/− mice not treated with dexamethasone, whereas in MIF−/− mice, leukocyte interactions were not further inhibited by dexamethasone. RU486 increased LPS-induced leukocyte adhesion and emigration to a similar extent in both wild-type and MIF−/− mice, indicating that endogenous GC exert a similar inhibitory effect on leukocyte trafficking in wild-type and MIF−/− mice. Both MIF deficiency and RU486 treatment reduced VCAM-1 expression, while neither treatment modulated expression of ICAM-1 or chemokines CCL2, KC and MIP-2. These results suggest that endogenous MIF and GC regulate leukocyte-endothelial cell interactions in vivo reciprocally but through predominantly independent mechanisms, and that the anti-inflammatory effect of MIF deficiency is comparable to that of exogenous GC.
登录
查看更多内容
影响因子：
82.9
作者：
Calandra, T;Echtenacher, B;Glauser, MP
通讯作者：
Glauser, MP
影响因子：
--
作者：
Lacey, D;Sampey, A;Morand, E
通讯作者：
Morand, E
影响因子：
7.3
作者：
Ajuebor, MN;Gibbs, L;Perretti, M
通讯作者：
Perretti, M
影响因子：
5.1
作者：
HIRASAWA, N;WATANABE, M;OHUCHI, K
通讯作者：
OHUCHI, K
巨噬细胞是巨噬细胞迁移抑制因子的重要且以前未被认可的来源。
影响因子：
15.3
作者：
Calandra, Thierry;Bernhagen, Juergen;Mitchell, Robert A.;Bucala, Richard
通讯作者：
Bucala, Richard