MOUSE ANTIBODY TO PHOSPHOCHOLINE CAN PROTECT MICE FROM INFECTION WITH MOUSE-VIRULENT HUMAN ISOLATES OF STREPTOCOCCUS-PNEUMONIAE
MOUSE ANTIBODY TO PHOSPHOCHOLINE CAN PROTECT MICE FROM INFECTION WITH MOUSE-VIRULENT HUMAN ISOLATES OF STREPTOCOCCUS-PNEUMONIAE
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DOI：
10.1128/iai.60.5.1957-1962.1992
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发表时间：
1992-05-01
影响因子：
3.1
通讯作者：
CRAIN, M
中科院分区：
文献类型：
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作者：
BRILES, DE;FORMAN, C;CRAIN, M
以前的研究已经证明，小鼠抗体磷酸胆碱（PC）可以保护小鼠免受致命的感染所造成的几个，但不是所有的，小鼠强毒实验室菌株的肺炎链球菌。由于先前研究中使用的肺炎球菌菌株是通过小鼠传播的，并且在人体外繁殖多年，因此不知道PC抗体是否能够保护小鼠免受S。新分离的肺炎链球菌。在本研究中，我们研究了PC的免疫球蛋白G（IgG）单克隆抗体（MAb）的能力，以防止感染的14个肺炎球菌菌株的荚膜类型3，4，6A，和6B的小鼠。从一组69个新鲜临床分离株中选出9个菌株作为小鼠最强毒力菌株。5个是小鼠通过的实验室菌株。针对PC的小鼠IgG3 MAb能够对几乎所有静脉注射菌株的感染和70%腹腔注射菌株的感染表现出保护作用（存活或死亡时间延长）。抗PC抗体的保护作用似乎部分依赖于荚膜类型。抗PC单克隆抗体对3型荚膜菌株最有效，对4型菌株最无效。对于3型和4型菌株，抗PC单克隆抗体通常可以保护静脉内注射的CFU数量大于腹腔内注射。对于荚膜型6A和6B菌株，情况正好相反。
Previous studies have demonstrated that mouse antibodies to phosphocholine (PC) can protect mice against fatal infection caused by several, but not all, mouse-virulent laboratory strains of Streptococcus pneumoniae. Because the pneumococcal strains used in previous studies had been mouse passed and were propagated for many years outside of humans, it was not known whether antibody to PC would be able to protect mice against S. pneumoniae freshly isolated from humans. In the present study, we examined the ability of an immunoglobulin G (IgG) monoclonal antibody (MAb) to PC to protect against infections in mice caused by 14 pneumococcal strains of capsular types 3, 4, 6A, and 6B. Nine of these strains were selected as the most virulent strains for mice from a group of 69 fresh clinical isolates. Five were mouse-passed laboratory strains. Mouse IgG3 MAb to PC was able to exhibit protective effects (survival or increased time to death) against infection with virtually all of the strains injected intravenously and against infection with 70% of the strains injected intraperitoneally. The protective effects of antibody to PC appeared to be partially dependent on capsular type. MAb to PC was most effective against capsular type 3 strains and least effective against type 4 strains. With type 3 and type 4 strains, MAb to PC could frequently protect against larger numbers of CFU injected intravenously than intraperitoneally. For capsular type 6A and 6B strains the reverse was true.